Detection of the CCR5-d32 Mutation in Patients Infected with Occult Hepatitis B

authors:

avatar Mohammad Kazemi Arababadi 1 , * , avatar Ali Akbar Pourfathollah 2 , avatar Abdollah Jafarzadeh 3 , avatar Gholamhossein Hasanshahi 4 , avatar Mohammad Reza Afrooz 5 , avatar Mahmood Hadadian 5

Assistant Prof of Immunology, Cellular and Molecular Research Center, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran
Prof, Dept of Immunology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
Associate Prof of Immunology, Cellular and Molecular Research Center, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran
Assistant Prof of Hematology, Cellular and Molecular Research Center, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran
BSC in Laboratory Sciences, Rafsanjan Blood Transfusion Organization, Rafsanjan, Iran
Zahedan Journal of Research in Medical Sciences: Vol.11, issue 2; e94401
published online: August 20, 2009
article type: Research Article
received: September 21, 2008
accepted: May 09, 2009

how to cite: Kazemi Arababadi M, Pourfathollah A A, Jafarzadeh A, Hasanshahi G, Afrooz M R, et al. Detection of the CCR5-d32 Mutation in Patients Infected with Occult Hepatitis B. Zahedan J Res Med Sci. 2009;11(2):e94401. 

Abstract

Background: Occult hepatitis B infection (OBI) is a form of viral hepatitis in which the HBV-DNA is present in blood while the HBsAg is undetectable. As differences in genetics and immunological responses have important role on OBI we were persuaded to analyze the known CCR5-D32 mutation (a chemokine receptor) in patients with OBI. Materials and Methods: In this descriptive study, 3700 blood samples that were HBsAg negative were collected during March 2006 to February 2007, from Rafsanjan blood transfusion services. All samples were tested for anti-HBc antibody. The HBsAg negative and anti-HBc positive samples were analyzed for HBV-DNA by PCR technique. The HBV-DNA positive samples were regarded as OBI cases. Finally, the Gap-PCR was performed to examine the d32 mutation in OBI patients. Results: The analysis of results showed that 352 (9.51%) cases of HBsAg negative samples were positive for anti-HBc. Following examination of HBsAg negative and anti-HBc positive samples for HBV-DNA by PCR based methods we found that 57(16.1%) cases had HBV-DNA. None of the OBI patients had d32 mutation in CCR5 chemokine receptor whereas 2(2%) of controls had heterozigotic form of this mutation. Conclusion: Based on the results of this study it seems that CCR5-D32 mutation does not affect the immune response against HBV to make OBI. Occult Hepatitis B Infection, CCR5, HBsAg, HBV-DNA, d32 Mutation

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