Effects of GLP-1 on γ-aminobutyric acid and serotonin metabolism in synaptosomal fractions from rat hypothalamus and brain stem

Mohsen Nikseresht; F Sanae; AA Owji; Hamdollah Delaviz

Zahedan Journal of Research in Medical Sciences

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Effects of GLP-1 on γ-aminobutyric acid and serotonin metabolism in synaptosomal fractions from rat hypothalamus and brain stem

Author(s):

Mohsen Nikseresht^1,*,

F Sanae²,

AA Owji³,

Hamdollah Delaviz⁴

1Biochemistry dept, Faculty of medicine, Yasuj University of Medical Sciences and health services, Yasuj, Iran.

2MS in biochemistry from Shiraz University of Medical Sciences and health services, Shiraz, Iran. Lecturer in Kazeroon Azad University.

3Biochemistry dept, Faculty of medicine, Shiraz University of Medical Sciences and health services, Shiraz, Iran.

4Anatomy dept, Faculty of medicine, Yasuj University of Medical Sciences and health services, Yasuj, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 6, issue 3; e94997

Published online:Sep 02, 2004

Article type:Research Article

Received:Jul 02, 2004

Accepted:Aug 03, 2004

How to Cite:Mohsen NiksereshtF SanaeAA OwjiHamdollah DelavizEffects of GLP-1 on γ-aminobutyric acid and serotonin metabolism in synaptosomal fractions from rat hypothalamus and brain stem.Zahedan J Res Med Sci.6(3):e94997.

Abstract

Background: Glucagons - like peptide-1 (7-36) amide (GLP-1) is the main product of the
glucagon gene expression in intestinal L cells. GLP-1 is released from the intestine into the
circulation in response to the ingestion of food and is the most potent stimulator of glucose-induced
insulin secretion. GLP-1 receptors have also been detected in discrete areas of rat brain and
intracerebro ventricular injection of GLP-1 has been shown to inhibit feeding in fasted rats.
Methods and Materials: This study is an in vitro study. Used rats are male and their weight is 200
to 220 grams and each group has 7 rats. In this study HPLC techniques were employed to evaluate
the effects of GLP-1 on monoamine metabolism in rat brain as a neuropeptide.
Synaptosoms were prepared from homogenates of combined hypothalamus and brain stem from
rats in each group. The synaptosomal pellets incubated for 15 minutes in ashaker bath at 37 C. The
effect of GLP-1 on synaptosoms was tested by adding 50 μL of GLP-1 5x10 –7 M to the incubation
medium. For this group a control group was prepared by adding normal saline instead of GLP-1.
The data were analyzed by T- test.
Results: GLP-1 decreased levels of serotonin (5-HT) by 20% (P<0.05) after 15 minutes of
incubation with combined hypothalamus and brain stem synaptosomes. Level of 5-
hydroxyindolacetic acid (5-HIAA), the principal metabolite of 5-HT, and tryptophan, the amino
acid precursor of 5-HT decreased by 21% and 37 %( P<0.05) respectively. γ-Aminobutyric acid
(GABA) and its amino acid precursor glutamic acid (Glu) were both quantities at the same
conditions as above , but a operculum derivatization HPLC technique was used. The increase levels
of GABA (14%) and Glu(6%) by GLP-1 was not significant .
Conclusions: The results suggest that decreased synaptosomal levels of 5-HT and 5-HIAA caused
by GLP-1 are due to diminished availability of tryptophan by the neuropeptide.

Keywords

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