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Jundishapur Journal of Natural Pharmaceutical Products
The Official Journal of Ahvaz Jundishapur University of Medical Sciences
The Effects of Different Media on Shoot Proliferation From the Shoot Tip of Aloe vera L.
Author(s):
1Department of Horticulture, Faculty of Agriculture, Ramin Agricultural and Natural Resources University, IR Iran
2Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz , IR Iran
*Corresponding Author: Department of Horticulture, Faculty of Agriculture, Ramin Agricultural and Natural Resources University, Ahvaz, IR Iran, [email protected]. Tel: +98-9163132702; +98-6123224334. Fax: +98-6123224350. Email: [email protected]
Published online:May 04, 2013
Article type:Research Article
Received:Mar 10, 2012
Accepted:Dec 23, 2012
How to Cite:Daneshvar MH, Moallemi N, Abdolah Zadeh N. The Effects of Different Media on Shoot Proliferation From the Shoot Tip of Aloe vera L.. Jundishapur J Nat Pharm Prod. 2013;8(2):. doi: https://doi.org/10.17795/jjnpp-4820
Abstract
Objectives:
In this study, the effect of different media on shoot proliferation of the shoot tip of
Materials and Methods:
In vitro techniques are some of the suggested methods for rapid propagation of Aloe. In this experiment, the shoot tips of mother plants were grown in a greenhouse. After surface sterilization of the explants, they were cultured on Murashige and Skoog (1962) (MS) medium containing different concentrations of kinetin and naphthaleneacetic acid (NAA). The experiment was carried out in the form of a randomized complete design with three replications.
Results:
The results showed that MS media containing 1.5 mg/L kinetin along with 0.15 or 0.3 mg/L NAA produced the highest percentage of proliferated shoots. In addition, the percentage of proliferated shoots in MS medium containing 2.0 or 2.5 mg/L benzylaminopurine (BAP) + 0.15 mg/L NAA was significantly higher than the other treatments.
Conclusions:
Analysis of the interactive effects of NAA, kinetin and BAP on shoot proliferation showed that most of the proliferated shoots produced in MS medium containing 1.0 mg/L BAP + 1.0 mg/L kinetin + 0.15 mg/L NAA were significantly different from other treatments. Rooting quality was greater in MS media containing 1.0 mg/L IBA than a 1.0 mg/L NAA treatment.
Copyright
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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