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Journal of Semnan University of Medical Sciences

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Cover image of the article: Assessment of Anti-Pseudomonal Potency of Honey Lactobacilli on the Drug-resistant Strains of Pseudomonas aeruginosa Isolated from the Burn Ward

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Outline

https://doi.org/10.69107/koomesh-155868

Assessment of Anti-Pseudomonal Potency of Honey Lactobacilli on the Drug-resistant Strains of Pseudomonas aeruginosa Isolated from the Burn Ward

Authors

Bent-Alhoda BarzegarBent-Alhoda Barzegar ORCID1, Leila FozouniLeila Fozouni ORCID1,*, Teena DadgarTeena Dadgar ORCID1
1Department of Microbiology, Go.C., Islamic Azad University, Gorgan, Iran
*Corresponding Author: Department of Microbiology, Go.C., Islamic Azad University, Gorgan, Iran. Email: lili_kia@yahoo.com
Koomesh:Vol. 27, issue 5; e155868
Published online:Sep 15, 2025
Article type:Research Article
How to Cite:Barzegar B, Fozouni L, Dadgar T. Assessment of Anti-Pseudomonal Potency of Honey Lactobacilli on the Drug-resistant Strains of Pseudomonas aeruginosa Isolated from the Burn Ward. Koomesh. 2025;27(5):e155868. doi: https://doi.org/10.69107/koomesh-155868

Abstract

Background: Pseudomonas aeruginosa is one of the most common gram-negative bacteria causing nosocomial and post-burn infections. Excessive use and abuse of antibiotics has led to the creation of multidrug-resistant bacteria and increased mortality in patients.

Objectives: This study was conducted to determine the prevalence of Ps. aeruginosa strains with multi-drug-resistant (MDR)/extensively drug-resistant (XDR) phenotypes and to investigate the inhibitory effects of honey lactobacilli on these isolates.

Methods: Using microbiological and biochemical tests, the Ps. aeruginosa strains were identified from 220 post-burn wound samples from hospitalized patients. The Kirby-Bauer test was performed to determine resistance phenotypes according to CLSI-2021 guidelines. After isolating thirteen types of honey probiotics by phenotypic and molecular methods, their antagonistic properties were evaluated by the disk diffusion method.

Results: A total of 63 (28.64%) isolates of P. aeruginosa were identified. Most positive samples of Ps. aeruginosa were isolated from patients with type 4 burns (38.09%). The results showed that 55.56% and 23.81% had MDR and XDR phenotypes, respectively. The highest sensitivity was observed to amikacin (60.32%). The results of disk diffusion showed that the average diameter of the non-growth zone caused by Lactobacillus plantarum (25 mm) on all isolates of MDR Ps. aeruginosa was 1.3-fold more than the probiotic effect of Lactobacillus brevis and 1.6 times more than the probiotic effect of L. acidophilus.

Discussion: Lactobacillus plantarum isolated from citrus fruits’ honey has a higher antibacterial potential on MDR P. aeruginosa isolates compared to other Lactobacillus species and other types of honey. More research is needed to confirm the effectiveness of honey lactobacilli for skin disinfection of hospitalized patients with burn infections.

 

 

Highlights

KeywordsDrug ResistancePseudomonas AeruginosaHoneyProbioticsBurns

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Copyright

© 2025, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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