Journal of Semnan University of Medical Sciences
Image Credit:Koomesh
Outline
The Anti-cancer Effect of the Methanolic Extract of Rhamnus cathartica on AGS and MNK45 Cell Lines In Vitro
Authors
Abstract
Background: Most of the basic drugs that are currently used by patients with different types of cancer have frequent side effects, so the search for a drug with strong anti-cancer effects and with low side effects is one of the goals of the World Health Organization (WHO) and medical centers in the treatment of cancer.
Objectives: The purpose of this study was to investigate the anticancer effect of Rhamnus cathartica methanolic extract on AGS and MNK45 cell lines.
Methods: Cancer cell lines were cultured in RPMI-1640 culture medium and after 48 hours and cell proliferation, 1×106 cells/mL were added to the wells of the 96-well plate. Rhamnus cathartica extract was added in concentrations of 100, 200, 400 and 800 µg/mL to each well and the lethality effect was evaluated using the MTT biochemical test.
Results: The results have shown that the methanolic extract of R. cathartica at concentrations of 200, 400 and 800 micrograms/mL is effective on AGS and MNK45 cell lines and at a concentration of 800 micrograms/ml, there is a significant difference compared to the group There was no positive control (P < 0.05).
Conclusion: Considering the lethality of the methanolic extract of R. cathartica on AGS and MNK45 cell lines, it can be concluded that the above compounds can be suitable candidates for the treatment of digestive cancers, of course, after additional studies.
Highlights
References
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© 2025, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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