Oral cavity that is surrounded by lips constitutes the 1/3 of the space between nose and chin. Lip corners are along with pupils. Sensory innervation of this area is supplied by maxillary and mandibular branches of the trigeminal nerve. Vermilion forms the major anatomic features of the lips. From external view, muscle fibers of the lip are separated from epidermis by subcutaneous tissue. Main blood supply of the lips include upper and lower branches of the facial artery, which enters into upper and lower lips and forms vascular ring of the vermilion. Vertical grooves over middle part of the upper lip constitute the philtrum. Vermilion tubercle fills the area beneath the philtrum in the middle part of the vermilion. Symmetric curvatures between middle part of the vermilion and its adjacent skin create a bowl shape view (
9). Philtrum is one of the most accurate landmarks for determination of the midline (
5). Mild dislocation of the philtrum from midline can lead to the considerable inelegance in eyes. Tonus and form of vermilion, vermilion tubercle, and philtrum are mainly influenced by maxilla bone, periodontal anatomy, and morphology and position of teeth. Slight alterations in the color and depth of the lip shadows vitalize the lips. Shadows and their depth determine the lip span. Lower lip that directly receives light seems shinier compared with upper lip. On the other hand, lower lip usually seems redder due to the great blood supply.
Distinguished features of the lower lip are not similar to the upper lip. There may be concavity or vertical groove in the middle area of the lower lip (
9). Labial frenum is a mucosal fold that contains muscle fibers and attaches to the lip. This natural anatomic structure has a role in lip movement. Labial frenum attachment can affect upper lip form and teeth visibility (
10). According to the result of a study, tonus of mentalis muscle decreases considerably following periodontal plastic surgery in patients with gummy smile and poor lip seal (
11). Normal lip expressions are influenced by race and physiology, but facial expressions in happiness, sorrow, anger, fear, wonder, hatred, and enthusiasm are likely similar in all cultures (
12,
13). In 1872, Charles Darwin published the first prominent study on the facial expressions. The results showed that main facial expressions including wonder, fear, shame, pride, hatred, horror, anger, love, joy, guilt, anxiety, timidity, and modesty are similar among people all over the world (
14).