Assessment of Bactaslyde media in diagnosis of Salmonella in water and foods in comparison with standard method in order to use in crisis

Hooman Motlagh; Hamidreza  Tavakoli; Arasb  Dabbagh Moghaddam; Narjes  Cheraghi; Hesameddin  Akbarein

Annals of Military and Health Sciences Research

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Assessment of Bactaslyde media in diagnosis of Salmonella in water and foods in comparison with standard method in order to use in crisis

Author(s):

Hooman Motlagh^1,*,

Hamidreza Tavakoli²,

Arasb Dabbagh Moghaddam³,

Narjes Cheraghi⁴,

Hesameddin Akbarein⁵

1Instructor, Department of Preventive Medicine, Golestan Hospital, AJA University of Medical Sciences, Tehran, Iran., Andorra

2Assistant Professor, Department of Nutrition and Food Hygiene, Baghiatollah University of Medical Sciences, Tehran, Iran, Andorra

3Instructor, Department of Social Medicine and Public Health, AJA University of Medical Sciences, Tehran, Iran, Andorra

4Researcher, Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, Andorra

5Researcher, Division of Epidemiology, Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, Andorra

Annals of Military and Health Sciences Research:Vol. 11, issue 2; e66215

Published online:May 06, 2013

Article type:Research Article

Received:Feb 19, 2013

Accepted:Apr 06, 2013

How to Cite:Hooman MotlaghHamidreza TavakoliArasb Dabbagh MoghaddamNarjes CheraghiHesameddin AkbareinAssessment of Bactaslyde media in diagnosis of Salmonella in water and foods in comparison with standard method in order to use in crisis.Ann Mil Health Sci Res.11(2):e66215.

Abstract

Background: Salmonellas are responsible for more than 40 percent of foodborne diseases in the world. Today, rapid diagnostic methods are essential for detecting these organisms in food and water especially in crisis. The aim of this study was to assess the Bactaslyde media usability in detection of salmonella and compare it with standard culture method.

Materials and Methods: In this laboratory study 20 samples of water and foods (pasteurized milk, water, raw Kabab and Raw Poultry Meat) were contaminated with salmonella standard strain (PTCC 1609) and then this salmonellas were detected by Bactaslyde culture media and standard culture method. Sensitivity, specificity, positive predictive value, negative predictive value and speed of two methods compared with each other.

Results: This study showed that Bactaslyde culture pouch number 4 can detect Salmonella in all 20 contaminated samples of food and water. The detecting time was 15 hours and 55 minutes in compare with 93 hours for standard method. Sensitivity of the test was 100%, specificity 90%, positive predictive value 90.91%, negative predictive value, 100% and validity of the test was 95%.

Conclusion: It was found that Bactaslyde culture method can detect Salmonella as a rapid test in all food and water samples and it can be recommended for using in crisis. More research with more samples and different foods is recommended.

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