Abstract
Background: Leishmaniasis is one of the health problems in. This study is designed to inhibit PG production by Indomethacin and induce NO by L- Arginine precursor in L. major infected Balb/c mice.
Materials and methods: This was an experimental study. Animals, Male inbred Balb/c mice were used in this study. The total number of animals used in this experiment was 48 Balb/c mice, divided into 6 groups (n =8 mice/group) including Group 1 (naive), Group 2 (L. major + 0.4% Ethanol), Group 3 (L. major + Indomethacin), Group 4 (L. major + DW), Group 5 (L. major + L- Arginine) and Group 6 (L. major + Indomethacin + LArginine). In addition to serum, liver and spleen suspensions were investigated for NO induction by using Griess microassay. The data was analyzed by Analysis of Variances (ANOVA) and Student’s t-test using Graph Pad Prism Software.
Results: The results indicated that production of NO was inhibited in infected Balb/c mice by L. major as compared with naive animals (Group 1). INDO as inhibitor PG (Group 2) showed their ability to elevate RNI levels in infect animals. INDO showed anti-leishmanial activity, as these compounds reduced the lesion sizes (P<0.001). INDO as inhibitor PG (Group 3) showed ability to decrease PG levels in infect animals.
Conclusion: Our data may indicate a possible role for L-Arg and INDO as novel drug targets for the treatment of cutaneous leishmaniasis in mouse model.
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© 2013, Annals of Military and Health Sciences Research. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.