Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER

Fatemeh Ghasemi; Hossein Nahrevanian

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Application of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER

Author(s):

Fatemeh Ghasemi^1,*,

Hossein Nahrevanian²

1Researcher, AJA University of Medical Science, Department of Biochemistry, Tehran, Iran, Andorra

2Resercher, Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran, Andorra

Annals of Military and Health Sciences Research:Vol. 11, issue 1; e66701

Published online:Jan 05, 2013

Article type:Research Article

Received:Sep 25, 2012

Accepted:Dec 05, 2012

How to Cite:Fatemeh GhasemiHossein NahrevanianApplication of L-Arginine as nitric oxide inducer and Indomethacin as prostaglandin inhibitor in Balb/c mice infected with Leishmania major MRHO/IR/75/ER.Ann Mil Health Sci Res.11(1):e66701.

Abstract

Background: Leishmaniasis is one of the health problems in. This study is designed to inhibit PG production by Indomethacin and induce NO by L- Arginine precursor in L. major infected Balb/c mice.

Materials and methods: This was an experimental study. Animals, Male inbred Balb/c mice were used in this study. The total number of animals used in this experiment was 48 Balb/c mice, divided into 6 groups (n =8 mice/group) including Group 1 (naive), Group 2 (L. major + 0.4% Ethanol), Group 3 (L. major + Indomethacin), Group 4 (L. major + DW), Group 5 (L. major + L- Arginine) and Group 6 (L. major + Indomethacin + LArginine). In addition to serum, liver and spleen suspensions were investigated for NO induction by using Griess microassay. The data was analyzed by Analysis of Variances (ANOVA) and Student’s t-test using Graph Pad Prism Software.

Results: The results indicated that production of NO was inhibited in infected Balb/c mice by L. major as compared with naive animals (Group 1). INDO as inhibitor PG (Group 2) showed their ability to elevate RNI levels in infect animals. INDO showed anti-leishmanial activity, as these compounds reduced the lesion sizes (P<0.001). INDO as inhibitor PG (Group 3) showed ability to decrease PG levels in infect animals.

Conclusion: Our data may indicate a possible role for L-Arg and INDO as novel drug targets for the treatment of cutaneous leishmaniasis in mouse model.

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