The synthesis of chitosan / polyethylene nanofibersoxide on chitosan membrane: an applicable model in tissue engineering

Background: Nanofibers as nano-scaffolds are widely used in tissue engineering. . Electrospun is a way to produce these fiberes. In this study, the synthesis of chitosan nanofibers / polyethylene oxide (CS / PEO) on a very thin membrane of CS as a basement membrane has been investigated.

Materials and Methods: For this purpose, composite solutions with volume fractions - density of CS and PEO, respectively, with ratios of 100 to zero (pure chitosan), 90 to 10 and 80 to 20 in Acetic Acid 5/0 M were prepared. Interactions of two polymers on each other were studied by Fourier transform infra-red spectroscopy (FTIR). Nanofiber diameter and size were studied with Scanning Electron Microscope (SEM). CS membrane polymer solution on the interval the electric field kv 20-18 cm 15-10 and feed rate ml / h 5/0 was electrospun. Nanofiber diameter and size were studied by Scanning Electron Microscope (SEM).

Results: FTIR study of the composite CS / PEO ratio of 20/80 and 10/90 peak tensile bond hydroxyl (OH) and (C = O) chitosan showed in certain areas. Scanning Electron Microscopic images showed that pure CS could not produce nanofibers on any ratios, and they appeared as a droplet. Whereas, nanofibers were created with ratios 80 to 20 and 90 to 10 of CS / PEO composite both on the aluminum foils and thin membrane of CS.

Conclusion: It seems that high viscosity of pure CS prevents nanofibers formation. Whereas, homogeneous nanofibers obtained from the composite of CS / PEO with the ratios 80 to 20, 90 to 10 and pure CS on thin membrane of CS.