Abstract
Background:
Iodine-131 therapy is one of the main strategies in the treatment of thyroid diseases such as hyperthyroidism and thyroid cancer. Iodine-131 emits high-energy beta particles and gamma rays. Although this technique is considered safe, there are many in vivo and in vitro studies reporting genotoxic damage induced by I-131. Induction of oxidative stress is one of the main mechanisms of therapeutic plan with I-131 and also mentioned as a side effect of it.Objectives:
The aim of this study was to determine the effect of Nano-curcumin on chromosomal damages in peripheral blood lymphocyte by micronuclei (MN) assay in patients with differentiated thyroid carcinoma (DTC) after therapeutic dose of I-131 as a clinical trial.Methods:
Twenty-one patients with DTC treated with I-131, activity (5.5 GBq) for destructing remnant thyroid functional tissue, were randomly selected to receive curcumin (nano-form, 160 mg/day for 10 days; n = 11) or placebo (n = 10) in a double-blind manner. We obtained blood samples from each patient before treatment and one week after radioiodine therapy. We calculated the number of MN in blood samples. The present study is the first clinical trial for investigating curcumin effectiveness to reduce genotoxicity effects by radiation after radioiodine therapy.Results:
The frequency of MN in lymphocyte was significantly increased at one week after I-131 treatment in placebo and curcumin groups. The frequency of MN in the curcumin group was significantly lower than placebo group after I-131 therapy. Nevertheless, there were no significant differences between both groups in White Blood Cell (WBC), Red Blood Cell, platelets, and hemoglobin level at before and after I-131 therapy.Conclusions:
The results of this study confirm that the administration of curcumin to the patients, in nano-micelle form, may prevent the genetic damage induced by I-131 in human lymphocyte.Keywords
Curcumin I-131 Differentiated Thyroid Carcinoma (DTC) Genotoxic Damage Micronuclei Assay
1. Background
Iodine-131 therapy has been known as one of the main strategies in the treatment of thyroid diseases such as hyperthyroidism and thyroid cancer (1). The most important therapy in patients with differentiated thyroid carcinoma (DTC) is thyroidectomy, usually followed by radiation therapy by I-131. Although this method is considered safe, it has some reported side effects. The therapeutic effect of iodine-131 is the result of beta and gamma radiations, which is damaging to cells with direct or indirect manner (2, 3). Most of Iodine-131 concentrates on residual thyroid tissue and kills tumor cells; however, it has several side effects such as radiation thyroiditis, sialadenitis, xerostomia, neck pain and edema, gastrointestinal symptoms (4), pulmonary problems, and defects in hematopoietic systems, nasolacrimal glands, and gonadal dysfunction, as well as second early malignancies (5, 6). The late complications like secondary cancers. These complications, seen after treatment with therapeutic dose of radioiodine (100 - 150 mCi), are rarely deadly, but they can affect the patient’s quality of life (7, 8). I-131 may also induce genetic damage and chromosomal instability in normal cells. Iodine 131 beta particles have the most important role in cytotoxic effects (9, 10). Ionizing radiation causes cellular damage mainly by producing reactive oxygen species (ROS), that can induce lipid peroxidation and damage to cell membranes and critical macromolecules such as DNA that lead to genetic damage (11, 12).
Recently, in vitro and in vivo studies showed radiosensitizing and radioprotective effects by some herbal chemicals and synthetic medications (13). One of these radioprotective photochemical is curcumin. Curcumin is the yellow polyphenol extracted from the rhizome of turmeric (Curcumalonga) (14). Curcumin has radioprotective, antioxidant, anti-inflammatory, anti-proliferative, anti-atherosclerotic, and pro-apoptotic effects in cancer cells (15). Curcumin prevents ROS forming and also scavenging free radicals and may protect the cells from toxicity that cause by reactive oxygen spices. Curcumin also increases the activities of antioxidant enzymes like superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px). Accordingly, curcumin may have beneficial effects against radiation toxicity by scavenging free radicals and produce antioxidant factors (16, 17). Oral curcumin is poorly absorbed from the bowel. However, the absorption of curcumin can be increased by newer formulations (i.e. complex with piperine, nanoparticles, micelles, liposomal formulations, etc.) (18). The use of nanoparticles as drug carriers has several advantages compared with another formulation. This nanoparticle can also cross the blood brain barrier (19).
Cytokinesis-block micronucleus assay in peripheral blood lymphocytes have been used for a long time as a biomarker of early effects of genotoxic carcinogens. Micronuclei (MN) are small, extra nuclear bodies that contain damaged chromosome/chromatid fragments (20). There are lots of studies that reported increase in the percentage of MN in peripheral lymphocytes of patients during radioiodine therapy (21-25).
Based on our knowledge and research, there is no study to report any effectiveness of nano-curcumin treatment in reducing the genotoxic damage in patients with DTC under radioiodine therapy. The aim of this study was to evaluate, in a double-blind placebo-controlled manner in patients with DTC after their first therapeutic dose of I-131, the effect of curcumin on complete blood counts with differential, platelet counts, and hemoglobin levels with chromosomal damages in peripheral blood lymphocyte by micronuclei assay.
2. Methods
This study was performed in 21 patients (11 women and 10 men, median age 44.95 years; range 24 - 79) (Table 1), who had undergone total thyroidectomy for DTC. None of the patients had previously been treated with external radiotherapy or I-131, and they did not suffer from any other illness except thyroid carcinoma without distant metastasis. This study was performed after obtaining permission from medical ethics committee of the university (Sbmu.rec.1393.797) and registered with Iranian registry of clinical trials (IRCT2016020526377N1). All patients provided written informed consent for participation.
Clinical Features of Patients with Differentiated Thyroid Carcinoma, Receiving Curcumin (160 mg/day) or Placebo at Before and After 131I Therapya,b
| Variables | Baseline (Before) | After 7 Days | ||
|---|---|---|---|---|
| Placebo (N = 10) | Curcumin (N = 11) | Placebo (N = 10) | Curcumin (N = 11) | |
| Age, ya | 46.6 (24 - 77) | 43.45 (27 - 56) | ||
| Sex, F/M | 5/5 | 6/5 | ||
| TSH, mIU/mL | 64.93 ± 22.4 | 52.38 ± 25.9 | ||
| TG, ng/mL | 9.93 ± 2.4 | 7.29 ± 2.6 | ||
| Anti TG, ng/mL | 5.15 ± 3.7 | 8.64 ± 4.2 | ||
| WBC, × 1000/mm3 | 7.42 ± 1.4 | 6.84 ± 0.7 | 7.41 ± 1.3 | 6.82 ± 0.7 |
| RBC, 106/µm3 | 5.10 ± 0.5 | 4.98 ± 0.5 | 5.13 ± 0.5 | 4.96 ± 0.5 |
| Platelet, × 1000/mm3 | 239.1 ± 51.98 | 276.81 ± 42.38 | 251.1 ± 44.5 | 275.45 ±43.32 |
| HGB, g/dL | 14.37 ± 1.9 | 14.75 ± 1.8 | 14.57 ± 1.8 | 14.80 ± 1.8 |
Randomization was performed in a double-blind fashion by administrative personnel outside the research project.
Eleven patients (6 women; median age 43.45 years, range 27 - 56) were assigned to receive nano-curcumin capsules; 10 patients (5 women; median age 46.6 years, range 24 - 77) received placebo (Table 1).
Nano-curcumin (Nano-micellar soft gel, red and flavorless capsules) was prepared from Elixir Nano Sina company (Iran). The details about synthesis and characterization of this drug have been reported in articles (26). A dose of 160 mg/day nano-curcumin or placebo was orally given at regular intervals from 3 days before up to 7 days after I-131 therapy. Patients received a thyroid remnant ablation dose of 5.5 GBq (150 mCi) I-131 (Box 1).
Inclusion/Exclusion Criteria
| Inclusion/Exclusion Criteria |
|---|
| Inclusion criteria: All patients with differentiated thyroid cancer (follicular, papillary or mixed type) not receiving destroying dose (ablative dose) of radioiodine up to now; no palpable neck mass; thyroid pathology reported with no invasion to capsular and lymph areas and blood vessels; body scan with I-131 not showing any functional tissue in regions other than the thyroid bed; thyroid cancer confirmed by biopsy; all patients have total thyroidectomy; TSH level before administration of radioiodine is higher than 30 IU/mL. |
| Exclusion criteria: After treatment with I-131, if there is abnormal tissue absorption of iodine in other areas of the thyroid bed; if patients after taking the first capsule of curcumin had a headache and dizziness. |
2.1. Evaluation of Blood Parameters
Blood samples (5 mL) were collected in heparinized sterile tubes from median cubital vein of patients before treatment and one week after radioiodine therapy. Complete blood counts with differential, platelet counts, and hemoglobin levels were obtained at baseline. Magnitude of changes from baseline in the hematological parameters was determined and compared. We also determined the blood parameters (serum thyroid stimulating hormone (TSH), thyroglobulin (TG), and anti-TG).
2.2. Micronucleus Assay
A 0.5 mL of blood sample (curcumin and placebo groups) was added to 4.5 mL of roswell park memorial institute (RPMI)-1640 culture medium (Gibco, USA), which contained 10% fetal calf serum, 2 mM glutamine (Gibco, USA), 0.1 mL/5 mL phytohemagglutinin (Gibco, USA), and antibiotics (Penicillin 100 IU/mL, Streptomycin 100 µg/mL) (Gibco, USA). Two paired cultures were set up for each blood sample and incubated at 37 ± 1°C. After 44 hours of culturing, Cytochalasin B (Sigma, final concentration: 5 µg/mL) was added to block cytokinesis induced by phytohemagglutinin. Cells were harvested at the end of 72 hours of incubation and extraction by centrifugation. They were treated by KCl 0.075 M and, then, fixed in fixative solution 3 times (methanol: acetic acid, 6:1). Next, we re-suspended and the suspension was dropped onto cold glass slides and allowed to dry. Fixed cells were stained with Giemsa (Merck, Germany) solution (10%). All slides were coded and checked at 40 × magnification for the micronuclei frequency in bi-nucleated cells with well-preserved cytoplasm. Counted micronuclei must have a diameter between 1/16th up 1/3rd of main nuclei, be non-refractile, and stick to or overlap with the main nuclei (27).
At each blood collection end point, at least 1000 bi-nucleated cells before and after radioiodine therapy cultures from each patient were examined to record the frequency of micronuclei.
2.3. Statistical Analysis
Statistical package (SPSS 16) was used for the data analysis. Data were expressed as mean ± SD unless otherwise stated and were tested for normal distribution (Shapiro-Wilk test). Paired sample t test was used for the comparison of micronuclei frequency in each group (curcumin and placebo) before and after therapy. We used independent sample t test to determine the difference between the frequency of MN between curcumin and placebo groups after radioiodine therapy. Statistical significance was assumed for P < 0.05.
3. Results
The demographic and biochemical characteristics of the patients are summarized in Table 1. At baseline, patients in the two groups (curcumin and placebo) were comparable for gender, age, serum TSH, thyroglobulin (TG), anti-TG, and the differences were non-significant. Based on the findings of this study, gender is unaffected in the frequency of micronuclei (12.1 ± 5.8 vs. 11.1 ± 2.5; P > 0.05) (Figure 1). Nano-curcumin administration was clinically safe and there was no evidence of side effects during our study.
Comparison the frequency of MN in patients in placebo and curcumin groups at baseline (before) and after radioiodine (131I) therapy. In vivo protection by curcumin against radiation-induced genetic damage induced by 131I (I) in whole blood lymphocyte. The data represent average ± standard deviation. At baseline, the mean frequency of peripheral blood lymphocyte MN was approximately the same between the two groups of patients and did not differ; comparison of the frequency of MN in patients after 7days of radioiodine therapy showed the significant differences (20.72 ± 7.1 vs. 31.5 ± 9.0; P = 0.006).
3.1. Micronucleus Assay
At baseline, the mean frequency of MN was approximately the same in both groups (10.81 ± 4.1 vs. 12.5 ± 4.5; P > 0.05) (Figure 1). The time course for the frequency of MN in human lymphocytes for each patient in the two groups is shown in Figure 1. The frequency of MN in the placebo group one week after I-131 treatment (31.5 ± 9.0; P = 0.000 vs. baseline) and the frequency of MN in curcumin treated group after 7 days was significantly increased compared to before I-131 therapy (20.72 ± 7.1; P = 0.001 vs. baseline) (Figure 1). The frequency of MN after one week of radioiodine therapy in curcumin subjects was significantly lower than placebo group (20.72 ± 7.1 vs. 31.5 ± 9.0; P = 0.006) (Figure 1). Total micronuclei frequencies were reduced by 32.4% in patients treated with curcumin compared to placebo group.
3.2. Evaluation of Blood Parameters
There was no significant difference between the two groups in terms of 4 parameters (White Blood Cell (WBC), Red Blood Cell (RBC), platelets, and hemoglobin level) at baseline and after 1 week (Table 1).
4. Discussion
Most patients with DTC are treated with I-131 after thyroidectomy to ablate remnant tissue (5). The TSH level in all patients before the administration of radioiodine was more than 30 IU/mL (Table 1). In some patients, the primary goal of the first dose of I-131 treatment is adjuvant therapy to decrease the risk of recurrence or treat persistent disease (28). Induction of oxidative stress is one of the main mechanisms operative in the therapeutic and/or side effects of iodine-131 (10). Monterio Gil et al. (26) investigated DNA damage induced by radioiodine therapy in peripheral blood lymphocytes in patients with thyroid cancer. The number of micronuclei in the cells increased after 1 month of treatment. Ballardin et al. (27) observed 4-fold increase in the frequency of micronuclei 7 days after radioiodine therapy in patients. In nuclear medicine, chromosomal damage following radioiodine therapy has been reported in many studies (29-31). It seems that at least 1 week after the administration of radioactive iodine-131 is a good time to evaluate cell damage in blood peripheral lymphocytes induced by radiation (30). In this study, we have also observed genotoxic effects in lymphocytes (2.52-fold increase) after 7days after radioiodine therapy.
The use of natural compounds to reduce the frequency of micronuclei induced by internal irradiation by radiation of I-131 is recently developed. Shafaghati et al. (1) incubated human lymphocytes with curcumin, at doses of 5, 10, and 50 µg/mL to investigate the radioprotective effects against the genotoxic damages in peripheral lymphocytes. After 1 hour, the lymphocytes were incubated with I-131 (100 µCi/1.5 mL) for two hours and maximum protective effects in micronuclei frequency were observed at the dose of 50 µg/mL curcumin with 52% reduction.
There are a limited number of clinical trials investigating the radioprotective effects of herbal chemicals on the side effects induced by radioiodine therapy. Based on our knowledge and researches, the present study for the first time as a clinical trial shows that nano-curcumin supplementation is effective in the reduction of genotoxic damages by radiation in patients with DTC. The current study confirms that I-131 therapy can induce a significant direct genotoxic damage characterized by the increase of MN in peripheral lymphocytes from patients with DTC in control groups. Dardano et al. (2) also showed this increase of MN (about 3 fold) in control groups without any radioprotectors materials. They have proved a significant protective effect of Ginkgo biloba extract (GBE) in patients with Graves’ disease and DTC receiving I-131 therapy for their treatments (2, 32). The results of the present research also confirm that the administration of curcumin to the patients, at the current dose, was able to prevent the increase of MN about 34.2% compared with the control group.
Several studies have found a relationship between antioxidant properties of curcumin and anti-oxidative stress in cells. Curcumin showed a strong capacity to scavenging intracellular small oxidative molecules such as H2O2, HO, and ROO. There are no reports of toxicity of curcumin in oral administration (32, 33). Curcumin plasma level range was from 29 to 419 ng/mL (33), and 22 to 42 ng/mL after 2 to 6 hours after single dose (32). Because of poor oral bioavailability of curcumin, some new studies focused on the formulation of this natural product in nano-micelles and water soluble to increase the absorption by intestine (32, 33). The administration of nano-curcumin showed the higher serum level of curcumin in blood and soft tissues. The half-life of curcumin in nano-micelles form in blood serum is about 6 to 7 hours after the first dose and the stability in the stomach is up to 72 hours; however, the peak of effectiveness is about 2 to 3 after single dose. The doses were selected on the basis of results from publication (34).
Bone marrow suppression is often a transient effect of radioiodine therapy. It shows decrease in WBC and platelet count for up to 6 to 10 weeks (35), but in this study, there were no significant differences between the two groups in terms of 4 parameters (WBC, RBC, platelets, and hemoglobin level) at baseline and after 1 week. We recommended that follow-up for 1, 3, and 6 months after therapy may show the differences.
4.1. Conclusions
The findings of this study show that curcumin may protect from possible oxidative and genotoxic damage induced by I-131 treatment. The main limitations of the current study were its sample size; so, further studies with larger cohorts of patients are needed to confirm this report. It should be underlined that the focus of interest of this study was to evaluate the radioprotective effects of curcumin after I-131 therapy, just in patients’ blood samples by the amount of MN frequency. But, for other effects, more follow-ups are needed.
Acknowledgements
References
-
1.
Shafaghati N, Hedayati M, Hosseinimehr SJ. Protective effects of curcumin against genotoxicity induced by 131-iodine in human cultured lymphocyte cells. Pharmacogn Mag. 2014;10(38):106-10. [PubMed ID: 24914274]. [PubMed Central ID: PMC4048555]. https://doi.org/10.4103/0973-1296.131020.
-
2.
Dardano A, Ballardin M, Caraccio N, Boni G, Traino C, Mariani G, et al. The effect of Ginkgo biloba extract on genotoxic damage in patients with differentiated thyroid carcinoma receiving thyroid remnant ablation with iodine-131. Thyroid. 2012;22(3):318-24. [PubMed ID: 22181338]. https://doi.org/10.1089/thy.2010.0398.
-
3.
Hosseinimehr SJ. Flavonoids and genomic instability induced by ionizing radiation. Drug Discov Today. 2010;15(21-22):907-18. [PubMed ID: 20933097]. https://doi.org/10.1016/j.drudis.2010.09.005.
-
4.
Bushnell DL, Boles MA, Kaufman GE, Wadas MA, Barnes WE. Complications, sequela and dosimetry of iodine-131 therapy for thyroid carcinoma. J Nucl Med. 1992;33(12):2214-21. [PubMed ID: 1460519].
-
5.
Van Nostrand D. The benefits and risks of I-131 therapy in patients with well-differentiated thyroid cancer. Thyroid. 2009;19(12):1381-91. [PubMed ID: 20001720]. https://doi.org/10.1089/thy.2009.1611.
-
6.
Jarzab B, Handkiewicz-Junak D, Wloch J. Juvenile differentiated thyroid carcinoma and the role of radioiodine in its treatment: a qualitative review. Endocr Relat Cancer. 2005;12(4):773-803. [PubMed ID: 16322322]. https://doi.org/10.1677/erc.1.00880.
-
7.
Tuttle RM, Becker DV, Hurley JR. Radioiodine treatment of thyroid disease. In: Sandler MP, Coleman RE, Patton JE, Wackers FJT, Gottschalk A, editors. Diagnostic nuclear medicine. 2003.
-
8.
Fard-Esfahani A, Emami-Ardekani A, Fallahi B, Fard-Esfahani P, Beiki D, Hassanzadeh-Rad A, et al. Adverse effects of radioactive iodine-131 treatment for differentiated thyroid carcinoma. Nucl Med Commun. 2014;35(8):808-17. [PubMed ID: 24751702]. https://doi.org/10.1097/MNM.0000000000000132.
-
9.
Baugnet-Mahieu L, Lemaire M, Leonard ED, Leonard A, Gerber GB. Chromosome aberrations after treatment with radioactive iodine for thyroid cancer. Radiat Res. 1994;140(3):429-31. [PubMed ID: 7972697]. https://doi.org/10.2307/3579122.
-
10.
Watanabe N, Kanegane H, Kinuya S, Shuke N, Yokoyama K, Kato H, et al. The radiotoxicity of 131I therapy of thyroid cancer: assessment by micronucleus assay of B lymphocytes. J Nucl Med. 2004;45(4):608-11. [PubMed ID: 15073256].
-
11.
Little JB. Radiation carcinogenesis. Carcinogenesis. 2000;21(3):397-404. [PubMed ID: 10688860]. https://doi.org/10.1093/carcin/21.3.397.
-
12.
Noaparast Z, Hosseinimehr SJ. Radioprotective agents for the prevention of side effects induced by radioiodine-131 therapy. Future Oncol. 2013;9(8):1145-59. [PubMed ID: 23902246]. https://doi.org/10.2217/fon.13.79.
-
13.
Garg AK, Buchholz TA, Aggarwal BB. Chemosensitization and radiosensitization of tumors by plant polyphenols. Antioxid Redox Signal. 2005;7(11-12):1630-47. [PubMed ID: 16356126]. https://doi.org/10.1089/ars.2005.7.1630.
-
14.
Hejazi J, Rastmanesh R, Taleban FA, Molana SH, Ehtejab G. A pilot clinical trial of radioprotective effects of curcumin supplementation in patients with prostate cancer. J Cancer Sci Ther. 2013;5(10):320-4. https://doi.org/10.4172/1948-5956.1000222.
-
15.
Jagetia GC, Rajanikant GK. Effect of curcumin on radiation-impaired healing of excisional wounds in mice. J Wound Care. 2004;13(3):107-9. [PubMed ID: 15045805]. https://doi.org/10.12968/jowc.2004.13.3.26589.
-
16.
Jagetia GC, Rajanikant GK. Acceleration of wound repair by curcumin in the excision wound of mice exposed to different doses of fractionated gamma radiation. Int Wound J. 2012;9(1):76-92. [PubMed ID: 21883936]. https://doi.org/10.1111/j.1742-481X.2011.00848.x.
-
17.
Ozgen SC, Dokmeci D, Akpolat M, Karadag CH, Gunduz O, Erbas H, et al. The Protective Effect of Curcumin on Ionizing Radiation-induced Cataractogenesis in Rats. Balkan Med J. 2012;29(4):358-63. [PubMed ID: 25207034]. [PubMed Central ID: PMC4115869]. https://doi.org/10.5152/balkanmedj.2012.038.
-
18.
Flora G, Gupta D, Tiwari A. Preventive efficacy of bulk and nanocurcumin against lead-induced oxidative stress in mice. Biol Trace Elem Res. 2013;152(1):31-40. [PubMed ID: 23292317]. https://doi.org/10.1007/s12011-012-9586-3.
-
19.
Yadav A, Lomash V, Samim M, Flora SJ. Curcumin encapsulated in chitosan nanoparticles: a novel strategy for the treatment of arsenic toxicity. Chem Biol Interact. 2012;199(1):49-61. [PubMed ID: 22704994]. https://doi.org/10.1016/j.cbi.2012.05.011.
-
20.
Mateuca R, Lombaert N, Aka PV, Decordier I, Kirsch-Volders M. Chromosomal changes: induction, detection methods and applicability in human biomonitoring. Biochimie. 2006;88(11):1515-31. [PubMed ID: 16919864]. https://doi.org/10.1016/j.biochi.2006.07.004.
-
21.
Hosseinimehr SJ, Shafaghati N, Hedayati M. Genotoxicity induced by iodine-131 in human cultured lymphocytes. Interdiscip Toxicol. 2013;6(2):74-6. [PubMed ID: 24179432]. [PubMed Central ID: PMC3798859]. https://doi.org/10.2478/intox-2013-0013.
-
22.
Gutierrez S, Carbonell E, Galofre P, Creus A, Marcos R. Cytogenetic damage after 131-iodine treatment for hyperthyroidism and thyroid cancer. A study using the micronucleus test. Eur J Nucl Med. 1999;26(12):1589-96. [PubMed ID: 10638411]. https://doi.org/10.1007/s002590050499.
-
23.
Ebrahimnejad P, Dinarvand R, Jafari MR, Tabasi SA, Atyabi F. Characterization, blood profile and biodistribution properties of surface modified PLGA nanoparticles of SN-38. Int J Pharm. 2011;406(1-2):122-7. [PubMed ID: 21185365]. https://doi.org/10.1016/j.ijpharm.2010.12.022.
-
24.
Fenech M. The in vitro micronucleus technique. Mutat Res. 2000;455(1-2):81-95. [PubMed ID: 11113469].
-
25.
American Thyroid Association Guidelines Taskforce on Thyroid N, Differentiated Thyroid C, Cooper DS, Doherty GM, Haugen BR, Kloos RT, et al. Revised American Thyroid Association management guidelines for patients with thyroid nodules and differentiated thyroid cancer. Thyroid. 2009;19(11):1167-214. [PubMed ID: 19860577]. https://doi.org/10.1089/thy.2009.0110.
-
26.
Monteiro Gil O, Oliveira NG, Rodrigues AS, Laires A, Ferreira TC, Limbert E, et al. Cytogenetic alterations and oxidative stress in thyroid cancer patients after iodine-131 therapy. Mutagenesis. 2000;15(1):69-75. [PubMed ID: 10640533]. https://doi.org/10.1093/mutage/15.1.69.
-
27.
Ballardin M, Gemignani F, Bodei L, Mariani G, Ferdeghini M, Rossi AM, et al. Formation of micronuclei and of clastogenic factor(s) in patients receiving therapeutic doses of iodine-131. Mutat Res. 2002;514(1-2):77-85. [PubMed ID: 11815246]. https://doi.org/10.1016/S1383-5718(01)00323-0.
-
28.
Nofal MM, Beierwaltes WH. Persistent Chromosomal Aberrations Following Radioiodine Therapy. J Nucl Med. 1964;5:840-50. [PubMed ID: 14247780].
-
29.
Dhillon N, Aggarwal BB, Newman RA, Wolff RA, Kunnumakkara AB, Abbruzzese JL, et al. Phase II trial of curcumin in patients with advanced pancreatic cancer. Clin Cancer Res. 2008;14(14):4491-9. [PubMed ID: 18628464]. https://doi.org/10.1158/1078-0432.CCR-08-0024.
-
30.
Kanai M, Yoshimura K, Asada M, Imaizumi A, Suzuki C, Matsumoto S, et al. A phase I/II study of gemcitabine-based chemotherapy plus curcumin for patients with gemcitabine-resistant pancreatic cancer. Cancer Chemother Pharmacol. 2011;68(1):157-64. [PubMed ID: 20859741]. https://doi.org/10.1007/s00280-010-1470-2.
-
31.
Tsai YM, Chien CF, Lin LC, Tsai TH. Curcumin and its nano-formulation: the kinetics of tissue distribution and blood-brain barrier penetration. Int J Pharm. 2011;416(1):331-8. [PubMed ID: 21729743]. https://doi.org/10.1016/j.ijpharm.2011.06.030.
-
32.
Zhongfa L, Chiu M, Wang J, Chen W, Yen W, Fan-Havard P, et al. Enhancement of curcumin oral absorption and pharmacokinetics of curcuminoids and curcumin metabolites in mice. Cancer Chemother Pharmacol. 2012;69(3):679-89. [PubMed ID: 21968952]. [PubMed Central ID: PMC3368221]. https://doi.org/10.1007/s00280-011-1749-y.
-
33.
Wahlang B, Kabra D, Pawar YB, Tikoo K, Bansal AK. Contribution of formulation and excipients towards enhanced permeation of curcumin. Arzneimittelforschung. 2012;62(2):88-93. [PubMed ID: 22344553]. https://doi.org/10.1055/s-0031-1295487.
-
34.
Silberstein EB, Alavi A, Balon HR, Clarke SE, Divgi C, Gelfand MJ, et al. The SNMMI practice guideline for therapy of thyroid disease with 131I 3.0. J Nucl Med. 2012;53(10):1633-51. [PubMed ID: 22787108]. https://doi.org/10.2967/jnumed.112.105148.
-
35.
Dardano A, Ballardin M, Ferdeghini M, Lazzeri E, Traino C, Caraccio N, et al. Anticlastogenic effect of Ginkgo biloba extract in Graves' disease patients receiving radioiodine therapy. J Clin Endocrinol Metab. 2007;92(11):4286-9. [PubMed ID: 17711926]. https://doi.org/10.1210/jc.2007-0597.