Detection of Somatic mutation of codon 248 of p53 gene between Iranian women with breast cancer

authors:

avatar Nasrin Shojaie 1 , * , avatar F. Tirgari 2

Department of Medical biochemistry, Iran Pasture Institute, Karaj, Iran
Department of Pathology, Imam Khomeini cancer Institute, Imam Khomeini hospital, Tehran, Iran
International Journal of Cancer Management: Vol.1, issue 1; e80392
published online: January 30, 2008
article type: Research Article
received: January 30, 2008
accepted: January 30, 2008

how to cite: Shojaie N, Tirgari F. Detection of Somatic mutation of codon 248 of p53 gene between Iranian women with breast cancer. Int J Cancer Manag. 2008;1(1):e80392. 

Abstract

Objective: Detection of P53 gene mutation on its codon 248 between Iranian women with breast cancer by PCR-RFLP.
Material and methods: DNA extraction of tumor tissues was performed in 53 patients with primary breast cancer. Designing primer for the sub-studying codon was performed with NTIVector software and detection of codon 248 mutation was done by Msp I PCR_RFLP.
Results: The somatic mutations of P53 on codon 248 were observed in 11.3% (6 of 53) of our cases, in which 9.4% were heterozygote mutation and the rest were homozygote mutation. This ratio is the highest reported percentage in the world.
Conclusion: This perusal indicates genotyping of P53 gene on codon 248 is important for Iranian women with breast cancer risk factors.

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