The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines

Mohsen Alipour; Seyed Jalal Zargar; Shahrokh Safarian; Shamileh Fouladdel; Ebrahim Azizi; Naser Jafargholizadeh

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

Outlines

Abstract
Copyright

The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines

Author(s):

Mohsen Alipour¹,

Seyed Jalal Zargar^1,*,

Shahrokh Safarian¹,

Shamileh Fouladdel²,

Ebrahim Azizi³,

Naser Jafargholizadeh¹

1Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

2Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 6, issue 2; e80399

Published online:Jun 30, 2013

Article type:Research Article

Received:Nov 20, 2012

Accepted:Feb 18, 2013

How to Cite:Mohsen AlipourSeyed Jalal ZargarShahrokh SafarianShamileh FouladdelEbrahim AziziNaser Jafargholizadehet al.The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines.Int J Cancer Manag.6(2):e80399.

Abstract

Background: The Bcl-2 protein family members have known as essential controllers of mitochondrial pathway of apoptosis. Bax is a proapoptotic member of Bcl-2 protein family, which is well known to play crucial roles for apoptosis control. bax has been implicated as potential tumor suppressor in certain solid tumors such as breast and colorectal carcinoma. DNA methylation of promoter associated CpG islands has known as a common mechanism for gene inactivation in tumor cells.

Methods: The Methylation Specific PCR (MSP) has used to find the methylation profile of the bax gene promoter CpG islands in colorectal and breast cancer cell lines.

Results: We have not detected any kind of "CpG islands hypermethylation" in promoter region of the bax gene in T47D, MCF7 (as ER positive), MDA-MB-231 and MDA-MB-468 (as ER negative) breast carcinoma-derived cell lines and colorectal cancer cell lines H29 and Caco II.

Conclusion: It seems that CpG island methylation could not play the main role in down-regulation of bax gene in breast and colon cancers.

Keywords

CpG island

DNA methylations

Polymerase Chain Reaction (PCR)

Breast cancer

Colorectal cancer

Fulltext

The Full text is available in PDF.

comments