The Investigation of Heat Shock Protein (HSP70) Expression Change in Human Brain Asterocytoma Tumor

Mehdi Pooladi; Sara Sobhi; Solmaz Khaghani Razi Abad; Mehrdad  Hashemi; Afshin Moradi; Ali Reza Zali; Masoumea Mousavi; Hakimeh Zali; Mona Zamanian Azodi; Azadeh Rakhshan; Mostafa  Rezaei Tavirani

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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The Investigation of Heat Shock Protein (HSP70) Expression Change in Human Brain Asterocytoma Tumor

Author(s):

Mehdi Pooladi¹,

Sara Sobhi²,

Solmaz Khaghani Razi Abad³,

Mehrdad Hashemi⁴,

Afshin Moradi⁵,

Ali Reza Zali⁶,

Masoumea Mousavi⁷,

Hakimeh Zali⁷,

Mona Zamanian Azodi⁷,

Azadeh Rakhshan²,

Mostafa Rezaei Tavirani^7,*

1Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences; Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehrans, Shahid Beheshti University of Medical Sciences, Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

4Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

5Cancer Research Center, Shahid Beheshti University of Medical Sciences; Dept. of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6Dept. of Neurosurgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran

7Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 6, issue Supplement; e80445

Published online:Dec 30, 2013

Article type:Research Article

Received:Nov 19, 2012

Accepted:Jan 14, 2013

How to Cite:Mehdi PooladiSara SobhiSolmaz Khaghani Razi AbadMehrdad HashemiAfshin MoradiAli Reza ZaliMasoumea MousaviHakimeh ZaliMona Zamanian AzodiAzadeh RakhshanMostafa Rezaei Taviraniet al.The Investigation of Heat Shock Protein (HSP70) Expression Change in Human Brain Asterocytoma Tumor.Int J Cancer Manag.6(Supplement):e80445.

Abstract

Background: Asterocytoma is the most common primary human brain tumor which has the most lethal hazard among tumors in human Central Nervous System. Heat Shock Proteins play an active role in cancer cells pathways through their effects on tumor cell proliferation, tumor differentiation, malignancy, metastasis, and cell death. HSP70 is a 70 KD protein that its expression change has been reported in various kinds of cancers. Here a proteomic activity for molecular diagnosis of Asterocytomas tumors is designed.

Methods: Proteins of tumor and normal brain tissues were extracted and then evaluated by Bradford test. In this study, the proteins were separated by 2DG Electrophoresis method, and then the spots were analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by Isoelectric point (pI), molecular weights and data banks.

Results: As a result, the 2D gel showed totally 800 spots. HSP70 is one of down regulated proteins.

Conclusion: As conclusion, alteration of HSP70 expression was detected in Asterocytomas which has an important role in tumor survival and development, response to cell stress and induces apoptosis in cells. Three kinds of HSP70 are known in different cancers: HSP70-1, HSP70-2 and HSP70-8 which affect limitation of apoptosis system in cancer cells.

Keywords

HSP70 Heat-Shock Proteins

Asterocytoma

Proteomics

Electrophoresis

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