Cell Survival Entropy and Cellular Resistance Activation Dose: Effect of Calprotectin on Gastric Adenocarcinoma Cell Line

Mostafa Rezaei‐Tavirani; Mohammad Rahmati‐Roodsari; Mehdi Mirzaie; Pooneh Amini Geram; Sara Sobhi

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Cell Survival Entropy and Cellular Resistance Activation Dose: Effect of Calprotectin on Gastric Adenocarcinoma Cell Line

Author(s):

Mostafa Rezaei‐Tavirani¹,

Mohammad Rahmati‐Roodsari²,

Mehdi Mirzaie³,

Pooneh Amini Geram¹,

Sara Sobhi^4,*

1Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4Cancer research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 6, issue Supplement; e80450

Published online:Dec 30, 2013

Article type:Research Article

Received:Dec 20, 2012

Accepted:Jan 18, 2013

How to Cite:Mostafa Rezaei‐TaviraniMohammad Rahmati‐RoodsariMehdi MirzaiePooneh Amini GeramSara SobhiCell Survival Entropy and Cellular Resistance Activation Dose: Effect of Calprotectin on Gastric Adenocarcinoma Cell Line.Int J Cancer Manag.6(Supplement):e80450.

Abstract

Background: The survival curves generally have been used for analysis of cell survival under stress conditions. They depict the relationship between the fraction of cells retaining their reproductive integrity and the absorbed dose. The median lethal dose, Lethal Concentration, 50% (LC50) of a toxin, radiation, or pathogen is the dose required to kill half the members of a tested population after specified test duration. LC50 frequently are used as a general indicator of a substance's acute toxicity. In this study a novel thermodynamic model is introduced to examine cell survival under stress condition. The survival function describes the surviving fraction of cell survival in different doses and contains information about cell survival. However, for interpretation of cells behavior under stress condition, thermodynamic formalism is utilized.

Methods: Gastric adenocarcinoma cells line were seeded, in the 96 well plates and incubated at 37 °C under 5% CO2 atmosphere for 48 hours. After that, the various concentrations of calprotectin were induced into cells for 48hours for MTT assay proposes. Viability data were analyzed by entropy function and correspond results was plotted. The statistical analysis applied for data validation.

Results: The entropy of survival function has a maximum value at LC50 and is asymmetric around LC50 value, and the rate of change of entropy function is different below and above LC50 value which indicates that cells have different behavior. The entropy function has a more gentle slope above LC50 so we can assume that living cells above LC50 are more resistant to poison used.

Conclusion: It can be concluded that regard entropy function and its derivations provides more possibility for revealing mechanism of cell behavior in stress conditions.

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