Rho GDP Protein Expression Change and Investigation of ItsEffect on GDP/GTP Cycle in Human Brain Oligodendroglioma

Solmaz  Khaghani Razi Abad; Mehdi Pooladi; Mehrdad Hashemi; Afshin Moradi; Sara Sobhi; Alireza Zali; Masoumea Mousavi; Hakimeh Zali; Mona Zamanian Azodi; Azadeh  Rakhshan; Reza Vafaee; Mostafa  Rezaei Tavirani

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Abstract
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Rho GDP Protein Expression Change and Investigation of ItsEffect on GDP/GTP Cycle in Human Brain Oligodendroglioma

Author(s):

Solmaz Khaghani Razi Abad¹,

Mehdi Pooladi¹,

Mehrdad Hashemi²,

Afshin Moradi³,

Sara Sobhi⁴,

Alireza Zali⁵,

Masoumea Mousavi⁶,

Hakimeh Zali⁶,

Mona Zamanian Azodi⁶,

Azadeh Rakhshan⁷,

Reza Vafaee⁶,

Mostafa Rezaei Tavirani^6,*

1Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3Dept. of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5Dept. of Neurosurgery, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

7Dept. of Pathology, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 6, issue Supplement; e80454

Published online:Dec 30, 2013

Article type:Research Article

Received:Jan 09, 2013

Accepted:Feb 12, 2013

How to Cite:Solmaz Khaghani Razi AbadMehdi PooladiMehrdad HashemiAfshin MoradiSara SobhiAlireza ZaliMasoumea MousaviHakimeh ZaliMona Zamanian AzodiAzadeh RakhshanReza VafaeeMostafa Rezaei Taviraniet al.Rho GDP Protein Expression Change and Investigation of ItsEffect on GDP/GTP Cycle in Human Brain Oligodendroglioma.Int J Cancer Manag.6(Supplement):e80454.

Abstract

Background: Oligodendrogliomas are sub-types of Gliomas with an enriched net of branching capillary. A vast amount of Oligodendroglial (approximately 60-90%) demonstrates a loss of 1p and 19q chromosomes, and their response to radio and chemotherapy treatments are considerably more favorable and less aggressive. Rho GDP inhibitor is a protein which controls the activity and distribution of GTPase in cells. In cancer cells with 1pLOH deletion, Rho GDP inhibitor protein can be anti-apoptotic. Here understanding of molecular diagnosis of Oligodendroglioma tumors is investigated via proteomic tools.

Methods: Proteins of tumor and normal brain tissues are extracted and separated by Two-Dimensional Gel (2DG) Electrophoresis method and the spots were then analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by pI, molecular weights and data banks.

Results: The 2D gels of normal and patient were provided and compared. As a result,high resolution analysis showed that there are totally 1328 spots in the gel. Bioinformatic analysis of the gels revealed that Rho GDI is one of the downregulated proteins.

Conclusion: It was revealed that Rho GDP inhibitor deactivates GTPProtein complex through disintegration of GTP from Rho proteins, leading to prevention of Rho GDP to return to its cycling.

Keywords

Rho GDP

Oligodendroglioma

Two-Dimensional electrophoresis

Proteomics

Brain tumors

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