Flow Cytometry Analysis of Rosa Damascena Effects on Gastric Cancer Cell Line (MKN45)

Hanieh Khatib; Mostafa Rezaei‐Tavirani; Saeed Heidari Keshel; Mona Zamanian Azodi; Roghiyeh Omidi; Mohsen Biglarian; Sara Sobhi

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Abstract
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Flow Cytometry Analysis of Rosa Damascena Effects on Gastric Cancer Cell Line (MKN45)

Author(s):

Hanieh Khatib¹,

Mostafa Rezaei‐Tavirani²,

Saeed Heidari Keshel²,

Mona Zamanian Azodi²,

Roghiyeh Omidi²,

Mohsen Biglarian²,

Sara Sobhi^3,*

1Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 6, issue Supplement; e80457

Published online:Dec 30, 2013

Article type:Research Article

Received:Nov 08, 2012

Accepted:Dec 25, 2012

How to Cite:Hanieh KhatibMostafa Rezaei‐TaviraniSaeed Heidari KeshelMona Zamanian AzodiRoghiyeh OmidiMohsen BiglarianSara Sobhiet al.Flow Cytometry Analysis of Rosa Damascena Effects on Gastric Cancer Cell Line (MKN45).Int J Cancer Manag.6(Supplement):e80457.

Abstract

Background: Gastric cancer is a life threatening disease. Recent studies have shown that Rosa Damascene possesses noticeable biological effects on the human body. This study, investigates the anticancer effect of Rosa Damascena on gastric cancer cell line MKN45.

Methods: Microscopic studies and MTT assay were used to examine morphological alteration, and cell survival of the cancer cells while exposing to differentvolumes of the essential oilrespectively. In addition, flow cytometry was applied to determine the cell death mechanism of the gastric cancer treated with the essential oil.

Results: The findings indicate that Rosa Damascena essential oil affects gastric cancer cells in two distinctive ways: the soluble phase increases cell viability, while the vapor phase decreases cellsurvival. Moreover, flow cytometry analysis revealed that apoptosis is the main mechanism accompanied with cell death.

Conclusion: Consequently, Rosa Damascena essential oil can be used as a potent anticancer agent in the future. However,more evaluation of this essential oil is still needed to elicit its effectivebiological activities

Keywords

Cell line

Essential oils

Rosa Damascene

Anticancer properties

Flow cytometry

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