The Effects of Aqueous Extract of Alpinia Galangal on Gastric Cancer Cells (AGS) and L929 Cells in Vitro

Mosa-Al- Reza Hadjzadeh; Habib Ghanbari; Zakieh  Keshavarzi; Jalil Tavakol-Afshari

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

Outlines

Abstract
Copyright

The Effects of Aqueous Extract of Alpinia Galangal on Gastric Cancer Cells (AGS) and L929 Cells in Vitro

Author(s):

Mosa-Al- Reza Hadjzadeh¹,

Habib Ghanbari¹,

Zakieh Keshavarzi^2,*,

Jalil Tavakol-Afshari³

1Dept. of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran

2Dept. of Physiology, North Khorasan University of Medical Sciences, Bojnurd, Iran

3Dept. of Immunology, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

International Journal of Cancer Management:Vol. 7, issue 3; e80543

Published online:Sep 30, 2014

Article type:Research Article

Received:Feb 10, 2014

Accepted:Jul 05, 2014

How to Cite:Mosa-Al- Reza HadjzadehHabib GhanbariZakieh Keshavarzi Jalil Tavakol-AfshariThe Effects of Aqueous Extract of Alpinia Galangal on Gastric Cancer Cells (AGS) and L929 Cells in Vitro.Int J Cancer Manag.7(3):e80543.

Abstract

Background: Although the incidence of gastric cancer is declining during the last half century, this cancer still is the second morbid cancer in the world after lung cancer. The incidence of gastric cancer is 26 per 100,000 in Iran. This study evaluated the effect of Alpinia galangal on AGS cells (human gastric adenocarcinoma epithelial cell line) and L929 cells (as a standard cell line originated from mouse fibroblast cells).

Methods: After culturing the cells in Roswell Park Memorial Institute (RPMI) medium, the cells were incubated with different doses of Alpinia galangal (0 (control), 125, 250, 500, 750 and 1000 µg/ml) in 24, 48 and 72 hour periods and then, cells viability were assessed using MTT based cell proliferation assay.

Results: After 24 hours, the percentage of living AGS cells compared to the control group showed no significant decrease at the concentrations of 125 and 250µg/ml. But in the rest concentrations were significant (p<0.05). Only, the percentage of surviving L929 cells at concentration of 125µg/ml of the extract was not significant, but these percentages in the other concentrations were significant. After 48 and 72h incubation, in the last three extract concentrations, the percentage of living AGS and L929 cells significantly decreased compared to control cells (p<0.05).

Conclusion: We have demonstrated, using cell culture model, anti-proliferative effect of aqueous extract of Alpinia galangal on human gastric tumor (AGS) and L929 cell lines. This effect was prominent in high concentrations.

Keywords

Fulltext

Full text is available in PDF.

comments