Further Stimulation of Cellular Immune Responses through Association of HPV-16 E6, E7 and L1 Genes in order to Produce more Effective Therapeutic DNA Vaccines in Cervical Cancer Model

Maryam Fazeli; Hoorieh Soleimanjahi; Simin Dadashzadeh

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

Further Stimulation of Cellular Immune Responses through Association of HPV-16 E6, E7 and L1 Genes in order to Produce more Effective Therapeutic DNA Vaccines in Cervical Cancer Model

Author(s):

Maryam Fazeli¹,

Hoorieh Soleimanjahi^1,*,

Simin Dadashzadeh²

1Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences Tehran, Iran

International Journal of Cancer Management:Vol. 8, issue 1; e80574

Published online:Feb 28, 2015

Article type:Research Article

Received:Nov 05, 2014

Accepted:Nov 29, 2014

How to Cite:Maryam FazeliHoorieh SoleimanjahiSimin DadashzadehFurther Stimulation of Cellular Immune Responses through Association of HPV-16 E6, E7 and L1 Genes in order to Produce more Effective Therapeutic DNA Vaccines in Cervical Cancer Model.Int J Cancer Manag.8(1):e80574.

Abstract

Background: Cervical cancer has been shown to be highly associated with human papillomavirus (HPV) infection. The viral oncogenes E6 and E7 are constantly expressed by the tumor cells and are therefore potent targets for therapeutic genetic vaccination. In the present study, it was investigated the potential effect of HPV-16 E6, E7 and L1 co-administration to activate specific cytotoxic T lymphocytes in tumor mice models.

Methods: The HPV-16 E6, E7 and L1 genes from Iranian isolate were separately inserted into the mammalian expression vector, pcDNA3, to construct the DNA vaccine candidates. Tumor-bearing Animals (C57BL/6 mice) were immunized with the vaccine candidate; then, Lymphocyte Proliferation Assay (LPA) and relative tumor volume measurements were carried out in order to examine the immunological effects of the vaccine.

Results: Obtained results showed that co-administration of the HPV-16 E6, E7 and L1 DNA induced HPV-16 specific cellular immune responses and also protected against TC-1-induced tumor in vivo compared with negative controls.

Conclusion: The results showed that mixed delivery systems might be valuable to improve the magnitude of the induced immune responses and confirmed therapeutic effects of HPV-16 E6, E7 through cytotoxic T lymphocyte induction and illustrate the new promising role for HPV-16 L1 CTL epitopes as a suitable CTL inducer.

Keywords

pcDNA3/E6

pcDNA3/E7

pcDNA3/L1

immunocellular responses

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