Purification of Hyaluronan Binding Proteins from Human Normal and Cancer Serum

M Siddaiah; B Fekry; KP Srinivas; RV Nunna; SD Banerjee; Karuna Kumar Mortha

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Abstract
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Purification of Hyaluronan Binding Proteins from Human Normal and Cancer Serum

Author(s):

M Siddaiah¹,

B Fekry¹,

KP Srinivas¹,

RV Nunna¹,

SD Banerjee²,

Karuna Kumar Mortha^1,*

1Department of Biochemistry, amounts of hyaluronan (hyaluronic acid, HA) when compared to human normal University of Mysore, Mysore, India

2Tufts University School of Medicine, Boston, United States

International Journal of Cancer Management:Vol. 3, issue 2; e80671

Published online:Jun 30, 2010

Article type:Research Article

Received:Mar 18, 2010

Accepted:Apr 27, 2010

How to Cite:M SiddaiahB FekryKP SrinivasRV NunnaSD BanerjeeKaruna Kumar Morthaet al.Purification of Hyaluronan Binding Proteins from Human Normal and Cancer Serum.Int J Cancer Manag.3(2):e80671.

Abstract

Background: Analysis of human cancer serum has revealed the presence of high amounts of hyaluronan (hyaluronic acid, HA) when compared to human normal serum, It is well documented that HA and its receptors, known as hyaladherins (HABPs) are involved in matrix regulation, cell proliferation, migration and at the matrix proper but also with hyaluronan at the plasma membrane as a cell surface receptors and thus influence cell physiology including secretion of this protein into the circulatory system.

Methods: Normal serum and colon cancer serum samples were included in this study using biochemical techniques such as gel permeation, strong anion exchange chromatography, single dimension electrophoresis and western blot analysis.

Results: This study is based on the clinical work of normal serum and colon cancer serum. The description of the procedure was given for the fractionation of serum proteins mainly HABPs from 20 normal and 15 colon cancer patients by using special biotinylated hyaluronan probe.

Conclusion: To evaluate whether serum HABPs levels could be used as diagnostic marker for human cancer. The semi purified serum from normal and colon cancer patients showed mainly a major protein (57kDa) and a minor one (30kDa) by overlay experiments with b-HA probe and these results were confirmed by competition experiments with cold HA.

Keywords

Hyaluronan (Hyaluronic acid

HA)

Hyaluronic acid binding protein (HABP)

Biotinylated hyaluronan probe (bHA)

Cold HA

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