Expression of H11 Antigen (HABP) in Benign and Malignant Cervical Tumor Tissue Using mAb H11B2C2

Siddaiah Manjunath; B Fekry; KK Mortha

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Abstract
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Expression of H11 Antigen (HABP) in Benign and Malignant Cervical Tumor Tissue Using mAb H11B2C2

Author(s):

Siddaiah Manjunath^1,*,

B Fekry¹,

KK Mortha¹

1Departmentof Biochemistry, University of Mysore, India

International Journal of Cancer Management:Vol. 3, issue 2; e80673

Published online:Jun 30, 2010

Article type:Research Article

Received:Jan 03, 2010

Accepted:Mar 20, 2010

How to Cite:Siddaiah ManjunathB FekryKK MorthaExpression of H11 Antigen (HABP) in Benign and Malignant Cervical Tumor Tissue Using mAb H11B2C2.Int J Cancer Manag.3(2):e80673.

Abstract

Background: The development and progression of human tumors is accompanied by interaction with extracellular matrix molecules including hyaluronan and hyaluronan binding protein (HA-HABP). Hyaluronan is a large polysaccharide associated with pericellular matrix of proliferating, migrating cells, regulation of hyaluronan expression during cervical ripening. Its implication in malignant transformation tumor progression and with the degree of differentiation in various invasive tumors has well accepted. It has been well known the role of HA receptors in tumor growth and metastasis in various cancer tissues.

Methods: The procedure carried out by using 10 benign and 30 different grades of cervical cancer tissue samples to see the expression of HABPs protein by western blotting method.

Results: This study is based on the clinical work of benign tissue and cervical cancer tissue samples. The results indicated that it is a HABP. It conforms that 57KD protein could be a common marker.

Conclusion: The HABP of 55-57KD and 30KD proteins, which were observed in western blot. It was shown by using a specific probe bHA for the detection of HABP, which recognizes all types of hyaladherins. Data suggests over-expression of H11 antigen (HABP) in tumor cells and it is an important parameter and a clinical diagnostic marker for all progressive human tumors.

Keywords

Hyaluronan (HA)

H11 antigen

Hyaluronan-binding proteins (HABP)

Cervical cancer

bHA (biotinylated hyaluronic acid)

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