Background: The development and progression of human tumors is accompanied by interaction with extracellular matrix molecules including hyaluronan and hyaluronan binding protein (HA-HABP). Hyaluronan is a large polysaccharide associated with pericellular matrix of proliferating, migrating cells, regulation of hyaluronan expression during cervical ripening. Its implication in malignant transformation tumor progression and with the degree of differentiation in various invasive tumors has well accepted. It has been well known the role of HA receptors in tumor growth and metastasis in various cancer tissues.
Methods: The procedure carried out by using 10 benign and 30 different grades of cervical cancer tissue samples to see the expression of HABPs protein by western blotting method.
Results: This study is based on the clinical work of benign tissue and cervical cancer tissue samples. The results indicated that it is a HABP. It conforms that 57KD protein could be a common marker.
Conclusion: The HABP of 55-57KD and 30KD proteins, which were observed in western blot. It was shown by using a specific probe bHA for the detection of HABP, which recognizes all types of hyaladherins. Data suggests over-expression of H11 antigen (HABP) in tumor cells and it is an important parameter and a clinical diagnostic marker for all progressive human tumors.
Hyaluronan-binding proteins (HABP)
bHA (biotinylated hyaluronic acid)
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