Prevalence of ETV6/RUNX1 Fusion Gene in Pediatric Patients with Acute Lymphoblastic Leukemia in Iran

Ahmad-Reza Rahnemoon; Farhad Zaker; Mina Izadyar; Shahla Ansari; Behzad Poopak; Yuri Tadavosyan

IJ Pediatrics

The Scientific Journal of Growth & Development Research Center

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Prevalence of ETV6/RUNX1 Fusion Gene in Pediatric Patients with Acute Lymphoblastic Leukemia in Iran

Author(s):

Ahmad-Reza Rahnemoon^1,*,

Farhad Zaker²,

Mina Izadyar³,

Shahla Ansari⁴,

Behzad Poopak⁵,

Yuri Tadavosyan⁶

IJ Pediatrics:Vol. 23, issue 6; 681-686

Published online:Dec 10, 2013

Article type:Research Article

Received:Nov 26, 2012

Accepted:Nov 16, 2013

How to Cite:Ahmad-Reza RahnemoonFarhad ZakerMina IzadyarShahla AnsariBehzad PoopakYuri Tadavosyanet al.Prevalence of ETV6/RUNX1 Fusion Gene in Pediatric Patients with Acute Lymphoblastic Leukemia in Iran.Iran J Pediatr.23(6):681-686.

Abstract

Objective: ETV6/RUNX1 (also known as TEL/AML1) is the most frequent gene fusion in childhood acute lymphoblastic leukemia (ALL). Sixty-three patients were enrolled in this study to explore the distribution of this gene in Iranian population. Methods: This study used 63 peripheral blood and bone marrow (PB/BM) samples from children with ALL. Immunophenotyping of PB and BM samples were performed using flow cytometry to illustrate the lineage. Moreover, reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to amplify transcripts of leukemia-specific chromosome fusion gene ETV6/RUNX1 and to monitor the expression levels of the ETV6/RUNX1 in patients according to Van Dongen et al protocol. Findings: On the basis of French-American-British (FAB) classification, 47 individuals had ALL-L1. The incidence of ETV6/RUNX1 fusion gene in this study was 34.9%. The laboratory and clinical features of twenty two ETV6/RUNX1 positive ALL cases were similar to those of other studies. The most positive cases of ETV6/RUNX1 fusion gene had the early pre B ALL and pre B ALL immunophenotypes. Conclusion: The ETV6/RUNX1 fusion gene is a common genetic anomaly in Iranian childhood ALL patients and the prevalence of the ETV6/RUNX1 fusion gene is similar to that of ALL patients in other countries. However early pre B cells were the most common type in studied patients.

Keywords

Acute Lymphoblastic Leukemia

Reverse Transcriptase

ETV6/RUNX1 Fusion

Polymerase Chain Reaction

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