Expression of Activation-Induced Cytidine Deaminase Gene in B Lymphocytes of Patients with Common Variable Immunodeficiency

asghar aghamohammadi; Hassan Abolhassani; Amir Salek Farrokhi; Shabnam Pourhamdi; Payam  Mohammadinejad; Bamdad Sadeghi; Seyed-Mohammad Moazzeni

IJ Pediatrics

The Scientific Journal of Growth & Development Research Center

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Expression of Activation-Induced Cytidine Deaminase Gene in B Lymphocytes of Patients with Common Variable Immunodeficiency

Author(s):

asghar aghamohammadi^1,*,

Hassan Abolhassani²,

Amir Salek Farrokhi³,

Shabnam Pourhamdi⁴,

Payam Mohammadinejad⁵,

Bamdad Sadeghi⁶,

Seyed-Mohammad Moazzeni⁷

IJ Pediatrics:Vol. 23, issue 4; 451-457

Published online:Jun 26, 2013

Article type:Research Article

Received:Nov 15, 2012

Accepted:Jun 04, 2013

How to Cite:asghar aghamohammadiHassan AbolhassaniAmir Salek FarrokhiShabnam PourhamdiPayam MohammadinejadBamdad SadeghiSeyed-Mohammad Moazzeniet al.Expression of Activation-Induced Cytidine Deaminase Gene in B Lymphocytes of Patients with Common Variable Immunodeficiency.Iran J Pediatr.23(4):451-457.

Abstract

Objective: Common variable immunodeficiency (CVID) is a heterogeneous disorder characterized by reduced serum level of IgG, IgA or IgM and recurrent bacterial infections. Class switch recombination (CSR) as a critical process in immunoglobulin production is defective in a group of CVID patients. Activation-induced cytidine deaminase (AID) protein is an important molecule involving CSR process. The aim of this study was to investigate the AID gene mRNA production in a group of CVID patients indicating possible role of this molecule in this disorder. Methods: Peripheral blood mononuclear cells (PBMC) of 29 CVID patients and 21 healthy controls were isolated and stimulated by CD40L and IL-4 to induce AID gene expression. After 5 days AID gene mRNA production was investigated by real time polymerase chain reaction. Findings: AID gene was expressed in all of the studied patients. However the mean density of extracted AID mRNA showed higher level in CVID patients (230.95±103.04 ng/ml) rather than controls (210.00±44.72 ng/ml; P=0.5). CVID cases with lower level of AID had decreased total level of IgE (P=0.04) and stimulated IgE production (P=0.02); while cases with increased level of AID presented higher level of IgA (P=0.04) and numbers of B cells (P=0.02) and autoimmune disease (P=0.02). Conclusion: Different levels of AID gene expression may have important roles in dysregulation of immune system and final clinical presentation in CVID patients. Therefore investigating the expression of AID gene can help in classifying CVID patients.

Keywords

Activation-Induced Cytidine Deaminase

AICDA Protein

Common Variable Immunodeficiency

Class Switching

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