Molecular Epidemiology of Rotavirus Strains Circulating among Children with Gastroenteritis in Iran

Akram Najafi; Maryam Zare; Mohammad Kargar

IJ Pediatrics

The Scientific Journal of Growth & Development Research Center

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Molecular Epidemiology of Rotavirus Strains Circulating among Children with Gastroenteritis in Iran

Author(s):

Akram Najafi¹,

Maryam Zare¹,

Mohammad Kargar^1,*

1Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

IJ Pediatrics:Vol. 22, issue 1; 63-69

Published online:Mar 31, 2012

Article type:Research Article

Received:Dec 24, 2010

Accepted:Sep 25, 2011

How to Cite:Akram NajafiMaryam ZareMohammad KargarMolecular Epidemiology of Rotavirus Strains Circulating among Children with Gastroenteritis in Iran.Iran J Pediatr.22(1):63-69.

Abstract

Objective: This study was conducted to evaluate the prevalence of rotavirus disease and to investigate the genotypes of rotavirus strains causing acute gastroenteritis among children aged <5 years old in Marvdasht, Iran.
Methods: One hundred and forty-one children, aged 1 month to 5 years, afflicted with severe diarrhea were enrolled during January 2007 to December 2008. Their stool samples were studied with enzyme immunoassays (EIA) for group A rotaviruses. Rotavirus-positive specimens were genotyped by the Nested RT-PCR using different types of specific primers.
Findings: Out of total collected samples rotavirus infection was detected in 40 (28.37%). Of the rotavirus episodes, 72.91% occurred during the first 2 years of life (P=0.038). The highest prevalence of infection was identified in summer (52.50%) and the lowest in winter (7.50%). The most common clinical features included diarrhea (96.25%), vomiting (82.50%) and fever (45.0%). Mixed genotypes were the predominant G type (60.0%), followed by non-typeable (12.50%), G2 (12.50%), G4 (10.0%) and G1 (5.0%) genotypes. G3/G8 mixed infection is the first of these rotavirus genotypes to be reported in Iran.
Conclusion: Regarding high frequency of rotavirus infection, continuous surveillance is needed to inform diarrhea prevention programs as well as to provide information about the occurrence of new rotavirus strains. This will assist policy makers in decision making on rotavirus vaccine introduction.

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