Objectives:
The following observation was primarily based on the study of gene fusion in blood and bone marrow cells taken from 68 Iranian children with acute lymphoblastic leukemia (ALL), to compare with healthy population.
IJ Pediatrics
The Scientific Journal of Growth & Development Research Center
RETRACTED ARTICLE: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children
Author(s):
1Allied Medical School, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IR Iran
2Children’s Medical Center, Pediatrics Center of Excellence, Tehran, IR Iran
3Department of Pediatrics, Tehran University of Medical Sciences, Tehran, IR Iran
4Department of Pediatrics, Iran University of Medical Sciences, Tehran, IR Iran
5Institute of Molecular Biology NAS RA, Yerevan, Armenia
Published online:May 29, 2018
Article type:Research Article
Received:Jan 12, 2016
Accepted:Jan 27, 2018
This article has been retracted. See Retraction Note: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children.
How to Cite:Ahmad Reza RahnemoonLeila KoochakzadehShahla AnsariAnna BoyajyanArsen ArakelyanRETRACTED ARTICLE: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children.Iran J Pediatr.28(3):e5371.https://doi.org/10.5812/ijp.5371.
Abstract
Methods:
Peripheral blood and bone marrow samples obtained from patients with ALL were immunophenotyped to determine the lineage and the level of differentiation. With reverse transcriptase-polymerase chain reaction (RT-PCR), the RNA molecules were analyzed according to Van Dongen et al. protocol to detect fused genes in cell population.
Results:
Leukemic cell type was identified by cytochemical stains and classified on the basis of FAB classification. Nonetheless the frequencies of E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR) and BCR/ABL(m-BCR) gene transcripts were 1.5%, 0%, 0% and 4.4% respectively. The positive case of E2A/PBX1 fusion gene had an early pre B and 3 BCR/ABL (m-BCR). Positive cases had an early pre B and pre-B ALL immunophenotype.
Conclusions:
Early pre-B cells were the most common types in our patients. The RT-PCR was shown to be an ideal method for detecting hybrid transcripts and to estimate the prevalence of the fusion genes in ALL patients. The frequency of these fusion genes in Iranian pediatric ALL patients were found to be similar to some developed countries. Thus, their presence does not seem to be predictive of increasing malignancy, but rather it can challenge the prognostic significance of these rearrangements.
Copyright
Copyright © 2018, Copyright Holder Demo. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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