Analysis of Genomic Diversity among Helicobacter pylori Strains Isolated from Iranian Children by Pulsed Field Gel Electrophoresis

Tahereh  Falsafi; Nazli  Sotoudeh; Mohammad-Mehdi  Feizabadi; Fatemeh  Mahjoub

IJ Pediatrics

The Scientific Journal of Growth & Development Research Center

Outlines

Abstract
References
Copyright

Analysis of Genomic Diversity among Helicobacter pylori Strains Isolated from Iranian Children by Pulsed Field Gel Electrophoresis

Author(s):

Tahereh Falsafi^1,*,

Nazli Sotoudeh²,

Mohammad-Mehdi Feizabadi³,

Fatemeh Mahjoub⁴

IJ Pediatrics:Vol. 24, issue 6; 703-70

Published online:Nov 28, 2014

Article type:Research Article

Received:Jan 02, 2014

Accepted:Nov 11, 2014

How to Cite:Tahereh FalsafiNazli SotoudehMohammad-Mehdi FeizabadiFatemeh MahjoubAnalysis of Genomic Diversity among Helicobacter pylori Strains Isolated from Iranian Children by Pulsed Field Gel Electrophoresis.Iran J Pediatr.24(6):703-70.

Abstract

Objective: Presence of genomic diversity among Helicobacter pylori (H. pylori) strains have been suggested by numerous investigators. Little is known about diversity of H. pylori strains isolated from Iranian children and their association with virulence of the strains. Our purpose was to assess the degree of genomic diversity among H. pylori strains isolated from Iranian-children, on the basis of vacA genotype, cagA status of the strains, sex, age as well as the pathological status of the patients. Methods: Genomic DNA from 44 unrelated H. pylori strains isolated during 1997-2009, was examined by pulse-field gel electrophoresis (PFGE). Pathological status of the patients was performed according to the modified Sydney-system and genotype/status of vacA/cagA genes was determined by PCR. PFGE was performed using XbaI restriction-endonuclease and the field inversion-gel electrophoresis system. Findings: No significant relationship was observed between the patterns of PFGE and the cagA/vacA status/genotype. Also no relationship was observed between age, sex, and pathological status of the children and the PFGE patterns of their isolates. Similar conclusion was obtained by Total Lab software. However, more relationship was observed between the strains isolated in the close period (1997-2009, 2001-2003, 2005-2007, and 2007-2009) and more difference was observed among those obtained in the distant periods (1997 and 2009). Conclusion: H. pylori strains isolated from children in Iran are extremely diverse and this diversity is not related to their virulence characteristics. Occurrence of this extreme diversity may be related to adaptation of H. pylori strains to variable living conditions during transmission between various host individuals.

Keywords

Fulltext

Click here to download PDF.

References

1.
Reference are available on the PDF.

comments