Isolation and Identification of E. cowanii from Powdered Infant Formula in NICU and Determination of Antimicrobial Susceptibility of Isolates

Jalal  Mardaneh; Mohammad-Mehdi Soltan-Dallal

IJ Pediatrics

The Scientific Journal of Growth & Development Research Center

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Isolation and Identification of E. cowanii from Powdered Infant Formula in NICU and Determination of Antimicrobial Susceptibility of Isolates

Author(s):

Jalal Mardaneh ¹,

Mohammad-Mehdi Soltan-Dallal^2,*

IJ Pediatrics:Vol. 24, issue 3; 261-266

Published online:May 09, 2014

Article type:Research Article

Received:May 19, 2013

Accepted:Nov 02, 2013

How to Cite:Jalal Mardaneh Mohammad-Mehdi Soltan-DallalIsolation and Identification of E. cowanii from Powdered Infant Formula in NICU and Determination of Antimicrobial Susceptibility of Isolates.Iran J Pediatr.24(3):261-266.

Abstract

Objective: Enterobacter cowanii is a genus of common gram-negative, facultatively anaerobic, rod-shaped, non-spore-forming bacterium of the Enterobacteriaceae family. This organism can be potentially a powdered infant milk formula-borne opportunistic pathogen. The aim of this study was to isolate and identify E. cowanii from consumed powdered infant formula milk (PIF) in intensive care units (NICU) and to determine antimicrobial susceptibility patterns of this bacterium.Methods: E. cowanii was isolated according to FDA method in 125 samples of PIF milk purchased from drug stores between Jun 2011 and March 2012. For final confirmation, biochemical tests embedded in API-20E system were used. The drug susceptibility test was performed using the disc diffusion method according to CLSI recommendations.Findings: Out of the 125 PIF samples investigated, 4 (3.2%) samples were positive for E. cowanii. All four isolates from PIF samples were uniformly susceptible to imipenem, meropenem, ceftazidime, ciprofloxacin, and colistin. Fifty percent of isolates were resistant to ampicillin, amoxicillin, and cotrimoxazoleConclusion: Analysis of the results indicated that complementary studies are necessary to clarify the possible role of E. cowanii as a food contaminant, in common NICU infections and high risk groups including persons with underlying disease and immunocompromised individuals.

Keywords

Powdered Infant Formula Milk

Neonatal Intensive Care Unit

E. Cowanii

Antimicrobial Susceptibility

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