Anti-oxidant and Anti-Inflammatory Cyclic Diarylheptanoids from Alnus japonica Stem Bark

authors:

avatar Sabrin R.M. Ibrahim a , b , * , avatar Gamal A. Mohamed c , d , avatar Amgad I. M. Khedr e , avatar Bader M. Aljaeid f

Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al Madinah Al Munawarah 30078, Saudi Arabia
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt
Department of Pharmacognosy, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Iranian Journal of Pharmaceutical Research : IJPR: Vol.16, issue Suppl; 83-91
article type: Original Article
received: April , 2015
accepted: July , 2015
DOI: https://doi.org/10.22037/ijpr.2017.1989

how to cite: R.M. Ibrahim S, A. Mohamed G, I. M. Khedr A, M. Aljaeid B. Anti-oxidant and Anti-Inflammatory Cyclic Diarylheptanoids from Alnus japonica Stem Bark. Iran J Pharm Res. 2017;16(Suppl):e124891. https://doi.org/10.22037/ijpr.2017.1989.

Abstract

A new cyclic diarylheptanoid namely alnuheptanoid B (3), along with four known cyclic diarylheptanoids: myricanone (1), (+)-S-myricanol (2), myricanone 5-O- -D-glucopyranoside (4), and (+)-S-myricanol 5-O- -D-glucopyranoside (5) were isolated from the EtOAc fraction of Alnus japonica Steud (family: Betulaceae) stem bark. Their structures were established by different spectroscopic analyses, as well as optical rotation measurement. Compounds 1, 2, 4, and 5 are isolated for the first time from A. japonica. The antioxidant and anti-inflammatory activities of compounds (1-5) were assessed using DPPH assay and carrageenin induced rat paw edema model, respectively. They displayed significant antioxidant activity in relation to propyl gallate (standard antioxidant) at concentration 50 µM. Compound 2 demonstrated anti-inflammatory effect at a dose 10 mg/kg compared with indomethacin (positive control).

Introduction

Diarylheptanoids have been isolated from various genera such as Acer (Aceraceae), Platycarya (Juglandaceae), Myrica (Myricaceae), Centrolobium (Leguminosae), Alpinia, Curcuma, and Zingiber (Zingiberaceae), and Alnus and Betula (Betulaceae) (1, 2). The structure of diarylheptanoids consists of two benzene rings linked by a linear C7-aliphatic chain with varying functional groups on the aryl and aliphatic moieties. They can be sub-grouped into open chain linear or cyclic compounds (2, 3). The latter group includes meta-meta bridged biphenyls and meta-para diphenyl ethers (2, 3). In addition, more complex diarylheptanoids with the basic skeleton extended by fragments such as arylbutyl, chalcone or flavonoid moieties have been isolated (4). They showed wide variety of biological activities as antioxidant, anti-inflammatory, antitumor, neuro-protective, estrogenic, hepatoprotective, anti-influenza, anti-trypanosomal, antiviral, and leishmanicidal (3, 5-15). In Japan, Alnus japonica (Betulaceae) is widely distributed in the low mountains (12, 13). Previous phytochemical study of A. japonica revealed the presence of diarylheptanoids, triterpenoids, flavonoids, and hydrolysable tannins (12-22). Continuing our study on A. japonica stem bark resulted in the isolation and characterization of alnuheptanoid B, a new cyclic diarylheptanoid, along with four known cyclic diarylheptanoids (Figure 1, Tables 1 and 2). They were assessed for their free radical scavenging activity using DPPH assay. Also, their anti-inflammatory effect was estimated using carrageenin induced paw edema method.

Experimental

General

Melting points were determined by Electrothermal Digital Melting Point 9100 instrument (England). Shimadzu 1601 UV/VIS and Shimadzu Infrared-400 (Japan) spectrophotometers were used to measure the UV and IR spectra, respectively. Optical rotation was measured by Perkin-Elmer Polarimeter 341 LC Model (USA). LRMS spectra were assessed by a MATTSQ7000 Finnigan spectrometer. A Micromass Qtof 2 spectrometer was used for HRESIMS spectra measurements. Bruker Avance DRX 400 (Bruker, USA) was used to record NMR spectra. HPLC separations were performed on a HPLC system consisting of a UV L-7400 detector (280 nm) and a Lachrom-Merck L-7100 Hitachi pump using a C18 column (250 × 8 mm i.d., Eurospher 100, Germany). A linear gradient (H2O:MeOH 80:20 % to MeOH 100 % over 45 min) was applied. Chromatographic separation was achieved using RP18 (0.04-0.063 mm) and silica gel (0.04-0.063 mm) 60 (Merck, Germany). The TLC analysis was performed using the following systems: MeOH:CHCl3 (10:90, S1), MeOH:CHCl3 (15:85, S2), and n-BuOH:H2O:HOAc (4:5:1, S3). Pre-coated silica gel 60 F254 TLC plates (0.2 mm, Merck) was used for TLC. Propyl gallate (PG), 2,2-diphenyl-1-picrylhydrazyl (DPPH), carrageenin, and indomethacin were provided by Sigma-Aldrich Co. (Taufkirchen, Germany).

Plant material

The plant sample was obtained from the Heinrich-Heine University`s botanical garden (Düsseldorf, Germany) in March 2005. The plant was taxonomically authenticated and identified by Peter Westhoff, Prof. of Plants Molecular Biology and Development (Heinrich-Heine University, Germany). A specimen (Registration code AJB-2005) was kept at the Faculty of Pharmacy, Department of Pharmacognosy, Al-Azhar University, Egypt.

Extraction and isolation

The powdered stem bark (200 g) was extracted exhaustively with EtOH (70 %, 4 × 2 L). The EtOH extract was concentrated to afford 12 g brown residue. The residue was chromatographed over vacuum liquid chromatography (VLC) using 50 % CHCl3:n-hexane (500 mL x 4) and EtOAc (500 mL x 4) to obtain 2.6 and 4.1 g, respectively. The VLC of the EtOAc (4.1 g) fraction using CHCl3:MeOH gradient elution afforded 6 sub-fractions (A-F). Sub-fractions B-E were previously investigated by authors (13). SiO2 column (2 cm 50 cm 50 g) of sub-fraction A (470 mg, CHCl3:MeOH 90:10 v/v) with CHCl3:MeOH gradient, followed by semi-preparative HPLC yielded 1 (22.1 mg, white needles) and 2 (14.8 mg, white needles). RP-18 column (2 cm 50 cm 60 g) of sub-fraction F (392 mg, CHCl3:MeOH 40:60 v/v) using MeOH:H2O gradient elution followed by HPLC gave 3 (13.7 mg), 4 (15.3 mg), and 5 (18.9 mg).

Myricanone (1)

White needles (22.1 mg); m.p. 191-192 °C; UV (MeOH) max: 215, 258, 295 nm; NMRdata: see Table 1; ESIMS: m/z 357 [M + H]+.

S-Myricanol (2)

White needles (14.8 mg); m.p. 103-104 °C; [α]D +38.5 (c 0. 5, CHCl3); UV (MeOH) max: 221, 259, 295 nm; NMR data: see Table 1; ESIMS: m/z 359 [M + H]+.

Alnuheptanoids B (3)

White amorphous powder (13.7 mg); UV (MeOH) max (log  ): 219 (4.71), 251 (4.36), 293 (3.95) nm; IR (KBr)  max: 3465, 1725, 1715, 1598 cm-1; NMR data: see Table 2; ESIMS: m/z 561 [M + H]+, 398.9 [(M + H)-Glu]+, 357.2 [(M + H)-(Glu+Acetyl)]+; HRESIMS: m/z 561.2339 (calc for C29H37O11, 561.2336 [M + H]+).

Myricanone 5-O--D-glucopyranoside (4)

White amorphous powder (15.3 mg); UV (MeOH) max: 220, 251, 294 nm; NMR data: see Table 2; ESIMS: m/z 519 [M + H]+, 357 [(M + H)-Glu]+.

(+)-S-Myricanol 5-O--D-glucopyranoside (5)

White amorphous powder (18.9 mg); [α]D +82.3 (c 0. 5, CH3OH); UV (MeOH) max: 231, 253, 294 nm; NMR data: see Table 2; ESIMS: m/z 521 [M + H]+, 359 [(M + H)-Glu]+.

Antioxidant activity

The antioxidant effect of compounds 1-5 was evaluated by 2,2`-diphenylpicrylhydrazyl (DPPH) assay as previously outlined (23, 24).

Carrageenin-induced rat paw edema method

The anti-inflammatory activity was evaluated on adult male albino rats (120-150 g b. wt.) using the same procedures as previously described (23, 25 ,26).

Statistical analysis

All data were expressed as mean ± standard error of mean using the student t test. ANOVA (one-way analysis of variance) was used for evaluation of statistical significance. The values were considered to be significantly different when P < 0.01.

Results and Discussion

Compound 3 was isolated as white amorphous powder. A molecular formula C29H36O11 was established from the HRESIMS quasi-molecular ion peak at m/z 561.2339 [M + H]+. The IR, UV, and NMR spectral data of 3 were in agreement with those of 4 except for the appearance of new signals at H 2.08 /C 20.8 (COCH3) and 171.7 (COCH3) characteristic for an acetyl group in 3. Its attachment at C-17 was confirmed by the 3J HMBC cross peak of H-16 to the carbonyl group of acetyl moiety at C 171.7 and further secured by the ESIMS ion peak at 357.2 [(M + H)-(Glu+Acetyl)]+. In addition, 3 was 42 mass units and one degree of unsaturation more than 4, confirming the presence of the acetyl moiety. The UV absorption maxima at 219, 251, and 293 nm indicated a diarylheptanoid structure of 3 (27). Its IR spectrum displayed bands ascribable to hydroxyl (3465 cm-1), ester carbonyl (1725 cm-1), ketone carbonyl (1715 cm-1), and benzene (1598 cm-1) functionalities (28). The 1H NMR spectrum showed two singlet methoxy groups at H 3.95 (4-OCH3) and 3.79 (3-OCH3) (Table 2). They correlated with the carbons resonating at C 61.9 (4-OCH3) and 61.7 (3-OCH3), respectively in HMQC spectrum. Their connectivity at C-4 and C-3 was proven by the HMBC cross peaks of 4-OCH3 to C-4 (C 145.0) and 3-OCH3 to C-3 (C 146.9). Four aromatic proton signals at H 7.06 (dd, J = 6.6, 1.5 Hz, H-15), 6.87 (d, J = 6.6 Hz, H-16), 6.69 (d, J = 1.5 Hz, H-18), and 6.67 (s, H-19), which correlated with carbons at C 129.6 (C-15), 122.8 (C-16), 132.4 (C-18), and 129.1 (C-19) in HMQC spectrum, indicating the presence of 1,2,4-tri-substituted and 1,2,3,4,5-penta-substituted benzene moieties in 3 (20,21). They were established by the COSY cross peaks of H-15 to H-16 and H-18 and further secured by the HMBC correlations of H-15 to C-17 and C-18, H-16 to C-1 and C-14, H-18 to C-14 and C-17, and H-19 to C-3 and C-5 (Figure 2). The connectivity of two phenyl moieties at C1-C2 was secured based on the HMBC cross peaks of H-19 to C-1 and H-18 to C-2 (Figure 2). Moreover, the doublet proton signal at H 4.80 (J = 7.6 Hz, H-1`) showed cross peak to the signal at C 105.0 (C-1`), indicating the presence of β-glucopyranose moiety (23, 26). This was established by the observed ESIMS fragment peaks at m/z 398.9 [(M + H)-Glu]+ and 357.2 [(M + H)-(Glu+Acetyl)]+. In the HMBC, the cross peak of H-1` to C-5 (C 148.7) established the placement of glucose at C-5. Furthermore, signals for six methylene groups at H 1.80-3.07 and ketone carbonyl at C 213.6 (C-11), characteristic for heptanoid moiety in 3 were observed. In the COSY spectrum, the spin system started from H-7 to H-10 and cross peak of H-12 to H-13 established this moiety. It was secured by the observed HMBC cross peaks of H-9/C-7 and C-11, H-10/C-8, H-12/C-13, and H-13/C-11. The attachment of heptanoid moiety at C6-C14 of the biphenyl moiety was secured by the HMBC cross peaks of H-8/C-6, H-19/C-7, and H-18/C-13. Consequently, 3 was concluded to be 17-O-acetyl myricanone 5-O-β-glucopyranoside and named alnuheptanoid B.

Compounds 1-5 were identified to be myricanone (1) (29, 30), (+)-S-myricanol (2) (30,31), myricanone 5-O- -D-glucopyranoside (4) (32), and (+)-S-myricanol 5-O- -D-glucopyranoside (5) (27) by the interpretation of the spectroscopic data and comparison with literature.

Table 1

1H and 13C NMR data of compounds 1 and 2 (400 and 100 MHz, CDCl3).

No.1
2
 H [m, J (Hz)] C (Mult.) H [Mult., J (Hz)] C (Mult.)
1-125.4 C-124.7 C
2-123.1 C-122.6 C
3-145.9 C-145.8 C
4-138.7 C-138.6 C
5-147.8 C-147.7 C
6-123.0 C-123.4 C
72.72 m26.8 CH22.55 m1.93 m25.7 CH2
81.94 m24.4 CH22.78 m1.92 m25.4 CH2
91.87 m21.8 CH21.69 m1.55 m23.0 CH2
102.77 m46.1 CH21.90 m1.54 m39.4 CH2
11-213.6 C4.08 m68.6 CH
122.81 m42.5 CH22.33 m1.72 m34.7 CH2
133.03 m28.8 CH22.94 m26.9 CH2
14-132.4 C-130.6 C
157.05 dd (6.6, 2.0)128.9 CH7.08 dd (7.0, 1.5)129.9 CH
166.88 d (6.6)116.9 CH6.91 d (7.0)116.8 CH
17-151.7 C-151.4 C
186.74 d (2.0)132.4 CH7.17 d (1.5)133.1 CH
196.60 s128.9 CH6.90 s129.4 CH
3-OCH33.81 s61.3 CH33.87 s61.3 CH3
4-OCH33.98 s61.4 CH33.99 s61.4 CH3
17-OH7.66 brs-7.70 brs-
OH5.91 brs-5.90 brs-
Table 2

1H and 13C NMR data of compounds 3-5 (400 and 100 MHz, DMSO-d6).

No.345
 H [m, J (Hz)] C (Mult.) H [Mult., J (Hz)] C (Mult.) H [Mult., J (Hz)] C (Mult.)
1-130.9 C-128.9 C-128.4 C
2-128.9 C-128.0 C-128.0 C
3-146.9 C-148.5 C-148.3 C
4-145.0 C-145.3 C-145.1 C
5-148.7 C-148.7 C-148.8 C
6-124.8 C-126.1 C-126.0 C
72.82 m2.72 m27.8 CH22.81 m27.1 CH22.54 m25.8 CH2
81.90 m1.86 m24.7 CH21.75 m24.2 CH22.71 m26.0 CH2
91.80 m21.9 CH21.51 m21.2 CH21.28 m1.21 m22.5 CH2
102.75 m2.67 m45.8 CH22.63 m45.1 CH21.63 m1.35 m39.3 CH2
11-213.6 C-213.2 C3.95 m66.5 CH
122.77 m42.4 CH22.74 m41.7 CH22.09 m1.49 m34.4 CH2
133.07 m2.95 m28.6 CH22.84 m28.0 CH22.83 m2.78 m26.8 CH2
14-132.1 C-130.7 C-129.4 C
157.06 dd (6.6, 1.5)129.6 CH6.95 dd (6.6, 1.7)128.3 CH6.96 dd (6.6, 1.5)129.1 CH
166.87 d (6.6)122.7 CH6.71 d (6.6)115.5 CH6.74 d (6.6)115.6 CH
17-149.8 C-152.3 C-152.0 C
186.69 d (1.5)132.4 CH6.45 d (1.7) 133.2 CH6.92 brs134.6 CH
196.67 s129.1 CH6.35 s128.5 CH6.60 s129.5 CH
1`4.80 d (7.6)105.0 CH4.79 d (7.6)103.4 CH4.84 d (7.6)103.9 CH
2`3.33 m74.1 CH3.19 m74.0 CH3.04 m74.0 CH
3`3.37 m77.2 CH3.06 m77.0 CH3.06 m77.1 CH
4`3.95 m69.9 CH3.16 m69.9 CH3.17 m69.9 CH
5`3.67 m76.3 CH3.24 m76.4 CH3.23 m76.5 CH
6`3.86 m3.70 m62.0 CH23.61 m3.43 m61.0 CH23.58 m3.42 m60.9 CH2
3-OCH33.79 s61.7 CH33.75 s60.1 CH33.81 s60.1 CH3
4-OCH33.95 s61.9 CH33.81 s60.9 CH33.83 s60.8 CH3
17-OH--8.91 brs---
2`-OH--5.03 brs-5.03 d (2.5)-
3`-OH--4.93 d (4.3)-4.93 d (4.1)-
4`-OH--4.09 d (4.3)-4.41 d (3.8)-
5`-OH--5.28 d (3.5)-5.22 d (3.5)-
6`-OH--4.34 t (4.6)-3.36 t (4.6)-
17-COCH32.08 s20.8 CH3171.7 C----

The antioxidant activity of the isolated cyclic diarylheptanoids (1-5) was determined using DPPH free radical scavenging system at concentration 50 µM. The results showed that, 1 and 2 had significant antioxidant activity. While, 3-5 showed moderate activity in comparison with propyl gallate (a known antioxidant) (Table 3). Their antioxidant effect was related to the number of free hydroxyl groups in their structures. Compounds 1 and 2 showed significant activities compared to propyl gallate at the same concentration. However, blocking of the hydroxyl group by an acetyl or glucose moiety leads to a decrease in the activity as in 3-5 (33).

Table 3

The DPPH radical scavenging activity results.

SampleDPPH (% Inhibition)
163.10 ± 0.81
270.14 ± 0.55
341.16 ± 0.64
449.09 ± 0.76
552.11 ± 0.59
Propyl gallate97.31 ± 0.37

Compounds 1-5 were evaluated for their anti-inflammatory effects using carrageenin induced paw edema model. Compound 2 showed the highest activity comparable to indomethacin (10 mg/kg) (Table 4). Also, 1, 3, 4, and 5 showed potent activity at dose 10 mg/kg after 4 h. The phenolic compounds are known to inhibit prostaglandins synthesis enzymes, more specifically the endoperoxide (26). It was reported that, prostaglandin like substances are released during the second phase of carrageenin induced edema (34, 35). So, the anti-inflammatory effects of the tested compounds may be due to inhibition of prostaglandin like substances.

Table 4

The anti-inflammatory activity results.

Groups n = 6Dose mg/kg0 hr
1 hr
2 hr
4 hr
6 hr
PETa% INbPETa% INbPETa% INbPETa% INbPETa% INb
Inflamed 3.95±0.090.005.76±0.120.006.51±0.110.006.97±0.100.004.88±0.130.00
Inflamed + Indom.c103.94±0.140.255.39±0.14*6.423.41±0.15*47.623.12±0.08*55.242.99±0.07*38.73
Inflamed + 1103.89±0.121.523.97±0.11*29.983.77±0.12*42.093.51±0.07*49.643.24±0.11*33.61
Inflamed + 2103.87±0.072.033.94±0.10*31.603.62±0.06*44.393.38±0.13*51.513.08±0.12*36.89
Inflamed + 3103.93±0.120.514.37±0.10*24.133.99±0.13*38.713.74±0.11*46.343.49±0.13*28.48
Inflamed + 4103.94±0.140.254.32±0.15*25.003.91±0.11*39.943.70±0.09*46.923.42±0.15*29.92
Inflamed + 5103.91±0.081.014.07±0.14*29.343.87±0.09*40.553.69±0.11*47.063.31±0.08*32.17

Significant different from inflamed control group at P < 0.01;

PET: Paw edema thickness;

% IN: % Inhibition;

Indom: Indomethacin.

The observed activity of these compounds might be through the inhibition of the inflammatory prostanoids (36, 37). In this work, we can make a conclusion on the SAR of the tested cyclic diarylheptanoids. It was observed that, the phenolic hydroxyl groups are responsible for anti-inflammatory activity as in 1 and 2 (36, 37). Glucosidation of phenolic OH group leads to reduce the activity as in 3-5. Secondary alcoholic hydroxyl group in aliphatic chain might increase the activity as in 1 and 5 in comparison to the other compounds.

Important 1H-1H COSY and HMBC correlations of alnuheptanoid B (3).

Important 1H-1H COSY and HMBC correlations of alnuheptanoid B (3).

Figure 2

Conclusions

A new cyclic diarylheptanoid and four known compounds were isolated from A. japonica for the first time. Their chemical structures were established by different spectroscopic analyses. Compounds 1 and 2 showed significant antioxidant activity. Compound 2 exhibited potent anti-inflammatory activity.

References

  • 1.

    Zhu J, Islas-Gonzalez G, Bois-Choussy M. Recent progress in isolation, bioactivity evaluation and total synthesis of diarylheptanoids. Org. Prep. Proced. Int. 2000;32:505-546.

  • 2.

    Lee YI, Lv H, She G. Naturally occurring diarylheptanoids. Nat. Prod. Commun. 2010;5:1687-1708. [PubMed ID: 21121274].

  • 3.

    Lv H, She G. Naturally occurring diarylheptanoids-A supplementary version. Rec. Nat. Prod. 2012;6:321-333.

  • 4.

    Prasain JK, Tezuka Y, Li JX, Tanaka K, Basnet P, Dong H, Namba T, Kadota S. Six novel diarylheptanoids bearing chalcone or flavonone moiety from seeds of Alpinia blepharocalyx. Tetrahedron. 1997;53:7833-7842.

  • 5.

    Lee MW, Kim JH, Jeong DW, Ahn KH, Toh SH, Surh YJ. Inhibition of cyclooxigenase-2 expression by diarrylheptanoids from the bark of Alnushirsuta var. sibirica. Biol. Pharm. Bull. 2000;23:517-518. [PubMed ID: 10784440].

  • 6.

    Shin D, Kinoshita K, Koyama K, Takahashi K. Antiemetic principles of Alpinia officinarum. J. Nat. Prod. 2002;65:1315-1318. [PubMed ID: 12350154].

  • 7.

    Agarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: preclinical and clinical studies. AnticancerRes. 2003;23:363-398.

  • 8.

    Kang GI, Kong PJ, Yuh YJ, Lim SY, Yim SV, Chun W, Kim SS. Curcumin suppresses lipopolysaccharide-induced cyclooxygenase-2 expression by inhibiting activator protein 1 and nuclear factor kappa B bindings in BV2 microglial cells. J. Pharmacol. Sci. 2004;94:325-328. [PubMed ID: 15037818].

  • 9.

    Jeong YS. Hepatoprotective and antioxidant effects of Alnus japonica extracts on acetaminophen-induced hepatotoxicity in rats. Phytother. Res. 2004;18:971-975. [PubMed ID: 15742342].

  • 10.

    Jin W, Cai XF, Na M, Lee JJ, Bae K. Diarylheptanoids from Alnus hirsuta inhibit the NF-kB activation and NO and TNF-α production. Biol. Pharm. Bull. 2007;30:810-813. [PubMed ID: 17409527].

  • 11.

    Park D, Kim HJ, Jung SY, Yook C, Jin CH, Lee YS. A new diarylheptanoid glycoside from the stem bark of Alnus hirsuta and protective effects of diarylheptanoid derivatives in human HepG2 cells. Chem. Pharm. Bull. 2010;58:238-241. [PubMed ID: 20118587].

  • 12.

    Wada H, Tachibana H, Fuchino H, Tanaka N. Three new diarrylheptanoid glycosides from Alnus japonica. Chem. Pharm. Bull. 1998;46:1054-1055.

  • 13.

    Ibrahim SRM, Fouad MA, Abdel-Lateff A, Okino T, Mohamed GA. Alnuheptanoid A: A new diarylheptanoid derivative from Alnus japonica. Nat. Prod. Res. 2014;28:1765-1771. [PubMed ID: 25116915].

  • 14.

    Tung NH, Kwon HJ, Kim JH, Ra JC, Ding Y, Kim JA, Kim YH. Anti-influenza diarylheptanoids from the bark of Alnus japonica. Bioorg. Med. Chem. Lett. 2010;20:1000-1003. [PubMed ID: 20045319].

  • 15.

    Tung NH, Suzuki M, Uto T, Morinaga O, Kwofie KD, Ammah N, Koram KA, Aboagye F, Edoh D, Yamashita T, Yamaguchi Y, Setsu T, Yamaoka S, Ohta N, Shoyama Y. Anti-trypanosomal activity of diarylheptanoids isolated from the bark of Alnus japonica. Am.J. Chin.Med. 2014;42:1245-1260. [PubMed ID: 25178281].

  • 16.

    Nomura M, Tokoroyama T, Kubotat T. Biarylheptanoids and other constituents from wood of Alnus japonica. Phytochem. 1981;20:1097-1104.

  • 17.

    Park J, Jeong HJ, Kim JH, Kim YM, Park S, Kim D, Park KH, Lee WS, Ryu YB. Diarylheptanoids from Alnus japonica inhibit papain-like protease of severe acute respiratory syndrome coronavirus. Biol. Pharm. Bull. 2012;35:2036-2042. [PubMed ID: 22971649].

  • 18.

    Kim HJ, Yeom SH, Kim MK, Shim JG, Paek IN, Lee MW. Nitric oxide and prostaglandin E2 synthesis inhibitory activities of diarylheptanoids from the barks of Alnusjaponica steudel. Arch. Pharm. Res. 2005;28:177-179. [PubMed ID: 15789747].

  • 19.

    Kuroyanagi M1, Shimomae M, Nagashima Y, Muto N, Okuda T, Kawahara N, Nakane T, Sano T. New diarylheptanoids from Alnus japonica and their antioxidative activity. Chem. Pharm. Bull. 2005;53:1519-1523. [PubMed ID: 16327181].

  • 20.

    Sati SC, Sati N, Sati OP. Bioactive constituents and medicinal importance of genus Alnus. Pharmacogn. Rev. 2011;5:174-183. [PubMed ID: 22279375].

  • 21.

    Lim SS, Lee MY, Ahn HR, Choi SJ, Lee J, Jung SH. Preparative isolation and purification of antioxidative diarylheptanoid derivatives from Alnus japonica by high-speed counter-current chromatography. J. Sep. Sci. 2011;34:3344-3352. [PubMed ID: 22083971].

  • 22.

    Lee M, Tanaka T, Nonaka G, Nishioka I. Dimeric ellagitannins from Alnus japonica. Phyroehtm. 1992;31:2835-2839.

  • 23.

    Mohamed GA, Ibrahim SRM, Al-Musayeib NM, Ross SA. New anti-inflammatory flavonoids from Cadaba glandulosa Forssk. Arch.  Pharm. Res. 2014;37:459-466. [PubMed ID: 24293064].

  • 24.

    Mohamed GA. New cytotoxic cycloartane triterpene from Cassia italica aerial parts. Nat. Prod. Res. 2014;28:976-983. [PubMed ID: 24684761].

  • 25.

    Mohamed GA, Abd-Elrazek AEE, Hassanean HA, Youssef DTA, van Soest R. New compounds from the Red Sea marine sponge Echinoclathria gibbosa. Phytochem.Lett. 2014;9:51-58.

  • 26.

    Mohamed GA, Ibrahim SRM, Elkhayat ES, Ross SA, Sayed HM, El-Moghazy SAM, El-Shanawany MA. Blepharisides A and B, new flavonol glycosides from Blepharis ciliaris growing in Saudi Arabia. Phytochem.Lett. 2015;11:177-182.

  • 27.

    Matsuda H, Morikawa T, Tao J, Ueda K, Yoshikawa M. Bioactive constituents of Chinese natural medicines VII) Inhibitors of degranulation in RBL-2H3 cells and absolute stereostructures of three new diarylheptanoid glycosides from the bark of Myrica rubra. Chem. Pharm. Bull. 2002;50:208-215. [PubMed ID: 11848211].

  • 28.

    (28) Silverstein RM, Webster FX. Spectrometric Identification of Organic Compounds. 6th ed. New York: John Wiley; 1998.

  • 29.

    Morihara M, Sakurai N, Inoue T, Kawai K, Nagai M. Two novel diarylheptanoids from Myrica gale var tomentosa and absolute structure of plant-chiral galleon. Chem. Pharm. Bull. 1997;45:820-823.

  • 30.

    Kawai S, Nakata K, Ohashi M, Tomoaki N. Myricanol and myricanone biosynthesis in Myrica rubra: incorporation of two molecules of 4-coumaric acid. J. WoodSci. 2008;54:256-260.

  • 31.

    Joshiat B. Extensive 1D, 2D NMR spectra of same [70] metacyclophanes and X-ray analysis of (±)-myricanol. J. Nat. Prod. 1996;59:759-764.

  • 32.

    Tao J, Morikawa T, Toguchida I, Ando S, Matsuda H, Yoshikawa M. Inhibitors of nitric oxide production from the bark of Myrica rubra: structures of new biphenyl type diarylheptanoid glycosides and taraxerane type triterpene. Bioorg. Med. Chem. 2002;10:4005-4012. [PubMed ID: 12413852].

  • 33.

    Dugas AJJ, Castañeda-Acosta J, Bonin GC, Price KL, Fischer NH, Winston GW. Evaluation of the total peroxyl radical-scavenging capacity of flavonoids: Structure-activity relationships. J. Nat. Prod. 2000;63:327-331. [PubMed ID: 10757712].

  • 34.

    Ibrahim SRM, Mohamed GA, Al-Musayeib NM. New constituents from the rhizomes of Egyptian Iris germanica L. Molecules. 2012;17:2587-2598. [PubMed ID: 22388969].

  • 35.

    Alcaraz MJ, Jimenez MJ. Flavonoids as anti-inflammatory agents. Fitoterapia. 1998;59:25-38.

  • 36.

    Bellik Y, Boukraâ L, Alzahrani HA, Bakhotmah BA, Abdellah F, Hammoudi SM, Iguer-Ouada M. Molecular mechanism underlying anti-inflammatory and anti-allergic activities of phytochemicals: An update. Molecules. 2013;18:322-353.

  • 37.

    Tatli II, Akdemir ZS, Yesilada E, Küpeli E. Anti-inflammatory and antinociceptive potential of major phenolics from Verbascum salviifolium Boiss. Z. Naturforsch. 2008;63c:196-202.