Abstract
Keywords
Premenstrual syndrome Wheat germ extract Menstrual disorders Herbal medicine
Introduction
Premenstrual syndrome (PMS) is a common disorder in women and affects millions of young women in reproductive ages (1-4). PMS symptoms begin 7-10 days before the start of the menstrual period, and fade away in the beginning days of mense (5-7). PMS symptoms fall into two domains: psychological and physical. Its most common psychological symptoms include tension, anxiety, crying, irritability, depression, hypersensitivity, poor concentration, fatigue, mood swings and anger. Its physical symptoms include breast tenderness, abdominal cramps, bloating, swelling, acne, increased appetite, headache, backache and sleep problems (8).
The exact cause of this syndrome is unknown (6,9), but the important causes include the reaction between the neuroendocrine system, mineral and vitamin (vitamins B and D) deficiency, increased aldosterone, reduced serotonin level, genetic background and different biologic and psychological factors have received attention (1,10-13). According to studies, 75% of women with regular menstrual cycles suffer PMS (2, 6, 14-16). PMS symptoms have low and medium severity in 20-32% of women (17); while they are so severe in 3-8% of women (18, 19) that interfere with their social relationships, work and daily life (11). Not using caffeine, alcohol, egg yolk, salty foods and excessive salt and little use of foods high in fat and protein, using skim milk and low-fat milk, high-carbohydrate foods, exercising regularly, eating fruits and vegetables and getting vitamins, thiamine and riboflavin from food sources may reduce symptoms of premenstrual syndrome(7,20). Recent findings show that a change in lifestyle, exercise, not consuming caffeine, alcohol, cigarette, using minerals and vitamins such as vitamins B6 and E, calcium, magnesium, medicines such as progesterone, diuretics, spironolactone, bromocriptine, prolactin inhibitors, serotonin reuptake inhibitors, anxiolytics, beta blockers, oral contraceptives, GNRH agonists, antidepressants, NSAIDS and herbal Medicine have been effective (11,21,22).
Herbal medicines are among the most common treatments because they are economical, safe and reliable, ease of application, non-invasive, and have fewer side-effects than do chemical medicines (23,24,25). Wheat germ, among herbal medicines, contains different kinds of vitamins, minerals and proteins, and is effective in treatment of diseases such as cancers, obesity, diabetes, asthma, anemia, eczema, hair loss, high blood pressure, ulcers and gastritis.
According to chemical analyses, wheat germ contains magnesium, zinc, calcium, selenium, sodium, potassium, phosphorus, chromium, antioxidants including beta-carotene (for vitamin A), vitamin E, vitamin C, vitamin B12, vitamin B6, thiamin, riboflavin, niacin, folic acid, iron, amino acids, and enzymes, and has a high dietary and medicinal value (26,27,28).
Various studies have been conducted on the positive effect of some wheat germ compounds (vitamins B6 and E, calcium, magnesium, essential fatty acids) on reducing PMS symptoms. Sabet Birjandi et al. have studied the positive effect of vitamin B6 on mitigating the physical and psychological symptoms (23). Also, Panay Moayyed studied the positive effect of vitamin B6 on reducing PMS symptoms (29). In other studies, positive effects of magnesium supplement for PMS symptoms have been addressed (30,31). Ghanbari et al. have pointed to the positive effect of calcium on reducing PMS symptoms (32). Furthermore, the positive effect of simultaneous intake of calcium and vitamin E on reducing more than 50% of PMS severity has been reported (33). Alexander has also confirmed the effectiveness of vitamin B6 on PMS (34). Linoleic acid has effectively reduced PMS symptoms, as well (33).
Given the positive effects of vitamins B6 and E, calcium, magnesium, essential fatty acids for reducing PMS symptoms, and the availability of all above compounds in wheat germ, the present study was conducted to examine the impact of wheat germ extract on PMS symptoms.
Experimental
Method
This triple-blind clinical trial was approved by the ethics committee of the university (reg. 116/2879), and registered in the clinical trial center (reg. IRCT201310286807N8). Then, a letter of introduction was obtained from the Shahid Beheshti University of Medical Sciences, the International Branch, and was presented to the head of the Hamedan University of Medical Sciences.
Having acquired the license from the university, we went to all the hospitals affiliated to Hamedan University of Medical Sciences. At the first stage, 624 employed women were interviewed from whom 170 entered the study based on the following inclusion criteria: 20-45 years of age, the body mass index of 19.8-26, having no night shifts, regular menstrual periods with 21-35 days cycles and 3-10 days bleeding period, no use of antidepressants, hormones and contraceptives, and vitamins in the past 3 months. They were excluded from the research in case of relatives’ death and divorce, adverse events, pregnancy, the use of 3 or less wheat germ extract capsules in two successive days, and the use of food supplements.
The participants, then, filled out the Beck Depression Inventory (BDI). If they got score 1-10, they entered the study. The Daily Symptom Record (DSR) and demographic questionnaire were presented to them, and they were briefed about how to complete them. Research units recorded daily symptoms severity with numbers 0 (no), 1 (mild), 2 (moderate) and 3 (severe). Then, mean severity of symptoms was calculated from 1 week prior to menstrual period to 3 days after the menstrual period. The percentage of the obtained average is severity of each symptom. Total severity of symptoms headache, breast tenderness, acne, swelling, bloating and palpitations demonstrates severity of physical symptoms, and total severity of symptoms irritability, tension, sleep problems, mood swings, food cravings, wish to be alone, depression, forgetfulness, anxiety, poor concentration, crying, depression, suicide, decreased libido and fatigue shows severity of psychological symptoms.
Also, a written consent form was taken from them. One hundred and thirty people filled out and returned the symptom daily recording form after 2 months, based on which the diagnosis of premenstrual syndrome was confirmed in 115 of them.
Of these, 15 people refused to participate and 100 people entered the study and were divided into two equal groups using the table of random numbers; 50 in the intervention and 50 in the control groups. After identification and verification of the wheat germ powder samples in the Botanical Laboratory at the School of Pharmacy at Shahid Beheshti University of Medical Sciences, first, the extraction was performed using ethanol 96% and repeated 3 times and each time for 24 hours. The dried extracts were used to produce capsules of wheat germ extract. 400 mg capsules of wheat germ extract or placebo were used three times a day in the intervention and control groups, respectively, between the 16th day of the menstrual cycle to the 5th day of the next menstrual period for two consecutive months. The questionnaire’s validity was assessed through content analysis. DSR and the PMS provisional diagnosis questionnaire are standardized worldwide, and their validity and reliability have been confirmed in previous studies (14, 35, 37).
BDI is also a global standard questionnaire whose validity and reliability have been confirmed in various studies (27, 37, 38). The data were analyzed in the SPSS-17 software. Friedman test was employed for comparison of three periods within groups, and Bonfaroni correction and the Mann Whitney test were used, respectively, pair comparisons within and between groups.
Results
Of the total 100 participants, 3 people were excluded for pregnancy, 3 for digestive complications, and 10 for missing or inappropriately taking the medicine. The length of the menstrual period in all participants was 3-10 days, and the interval between the periods was 21-35 days. The history of premenstrual syndrome in the wheat germ extract and in the placebo groups was 80.95% and 66.66%, respectively. On average, 73.80% of people with a positive family history of PMS experienced this syndrome.
In order to control the confounding factors, both groups were matched before intervention for age, body mass index, income, job, education level, menarche age, marital status, family history of PMS, and there was no statistically significant difference between the two groups (Table 1). As Tables 2, 3, and 4 shows, the two groups were matched for the severity of the general, physical and psychological symptoms, and there was no statistically significant difference between them in this respect.
Demographic characteristics of the 2 groups
| Treatment Categories | ||
|---|---|---|
| Variable | Wheat Germ Extract | Placebo |
| Age(years) | 34.9 ± 6.6 | 33.8 ± 6.5 |
Comparison of mean severity of physical& psychological &general symptoms premenstrual syndrome before and after the intervention in both groups.
| p-value | 8 weeks after treatment | 4 weeks after treatment | Before treatment | Treatment round groups |
|---|---|---|---|---|
| physical symptoms | ||||
| p < 0.001 | 10.48 ± 7.07 | 15.48 ± 8.87 | 28.79 ± 9.08 | Wheat Germ Extract |
| psychological symptoms | ||||
| p < 0.001 | 10.53 ± 5.41 | 15.55 ± 7.56 | 31.25 ± 13.69 | Wheat Germ Extract |
| general symptoms | ||||
| p < 0.001 | 10.50 ± 5.12 | 15.51 ± 6.54 | 30.02 ± 9.88 | Wheat Germ Extract Placebo |
The mean reduction in physical symptoms in the wheat germ extract and the placebo groups was 63.56% and 14.35%, the mean reduction in psychological symptoms in the wheat germ extract and the placebo groups was 66.30% and 27.43%: and the mean reduction in general symptoms in the wheat germ extract and the placebo groups was 64.99% and 21.05%, respectively (Table 2). The severity of the general and psychological symptoms had reduced in both groups, but this reduction was significantly greater in the wheat germ extract group (p<0.001) (Figure 1); while the reduction in severity of physical symptoms was statistically significant only in the wheat germ extract group (p<0.001), and there was not a statistically significant difference in the placebo group.
The greatest reduction in severity of symptoms after two months of treatment occurred for the following in order: fatigue (85.32%), irritability (84.80%), palpitation (80.24%), tension (80.24%), breast tenderness (79.71%), headache (76.52%), sleep problems (73.79%), increased appetite (73.12%), acne (70.40%), mood swings (69.64%), food cravings (68.71%), wish to be alone (68.10%), depression (61.64%), forgetfulness (59.15%), anxiety (58.94%), poor concentration (56.72%), crying (44.53%) and swelling (25.72%).
The Friedman test in the wheat germ extract group pointed to statistically significant differences in all symptoms (p<0.001) except the reduced desire for suicide, decreased severity of bloating and decreased libido. Neither of the groups showed changes in the duration and extent of bleeding, and there was not any statistically significant difference between the groups in this respect. The number of consumed painkillers remarkably reduced after intervention in the wheat germ extract group (p<0.001); while there was not a statistically significant difference in the placebo group on this score (Figure 2). Complications were not reported in 95.2% of the wheat germ extract group and 92.9% of the placebo group.
Discussion
The results showed that wheat germ extract significantly reduced the severity of general, physical and psychological symptoms of PMS. The reduction in severity of general and psychological symptoms was also statistically significant in the placebo group, but this reduction was significantly higher in the wheat germ extract group, showing that the reduction in the placebo group is probably caused by the psychological effect of the medicines. The results of a study by Ozgoli et al., also confirm that placebos have positive impacts on the reducing PMS symptoms (36), which is in accordance with the present study.
There is no research directly addressing the effect of wheat germ extract on PMS symptoms. There are some notable studies on the effect of wheat germ compounds (such as calcium, magnesium, vitamins D, B6 and E). The positive effect of vitamin B6 on reducing the symptoms of depression, anxiety, irritability, breast tenderness, and the superior effect of vitamin B6 compared to placebo’s have been shown (39). Another study revealed the positive impact of vitamin B6 on all PMS symptoms (16, 41). Also, the decreased score of PMS symptoms due to supplementary zinc and vitamin B6 has been reported (35). All the three studies are in line with the present study, which might be due to the effect of vitamin B6 found in wheat germ. The positive impact of vitamin E on reducing PMS symptoms, such as anxiety and breast tenderness has been revealed, which accords with our study. This similarity is justified through the effect of the vitamin E available in wheat germ on prostaglandin synthesis and the regulation of central neurotransmitters (41).
Although the main cause of PMS is not identified (6,9), the bulk of available evidence underlines the effect of ovarian hormones cyclic changes on central neurotransmitter mechanisms as the cause of the recurrent symptoms (4,14). Based on this, the role of B vitamins in wheat germ in regulating psychological status and mood imbalance, in particular depression in PMS, can be justified through the following mechanisms: the production of serotonin and tryptophan metabolism, pyridoxine-phosphate (the active form of the vitamin B6) in the proper synthesis of many neurotransmitters (42). The zinc in wheat germ might react with neurotransmitters which affect psychological balance (4), and reduce the symptoms. Wheat germ has sedative properties and is effective in the treatment of diseases such as ataxia, nervous system diseases and acute mental illnesses (20, 26). The iron in wheat germ is a required cofactor for the tryptophan hydroxylase. Also, the role of iron has been reported in the metabolism of serotonin and GABA. The symptoms of iron deficiency include depression, physical activity disorder, and cognitive problems, and the relationship between receiving iron and reduction in PMS symptoms has been reported (4).
Daily intake of 1000-1200 mg of calcium has proved effective in reducing symptoms of cramp, pain, fluid retention, flatulence, appetite changes and negative attitudes (16). Also, the benefit of the daily consumption of 1200 mg calcium for reducing tension, anxiety, swelling, and the desire to eat sweets has been shown (43); all the above mentioned studies are in agreement with our research, which might be due to the impact of calcium in wheat germ. The daily intake of 1000 mg calcium with 400 IU vitamin E reduces 63% of the psychological, 55% of the physical, and 49% of the behavioral symptoms. The daily use of 500 mg calcium reduces 50% of the general symptoms in 55% of people, and 75% of the symptoms in 30% of people after three months of intervention (44), which is in accordance with our study and might be due to the effect of calcium and vitamin E in wheat germ. The positive effect of magnesium consumption for reducing fatigue severity, palpitation, headache, appetite change, swelling, chest pain, bloating, anxiety, mood swings, forgetfulness, sleep disorders, frequent crying and tension has been documented, which is compatible with our study, and might be the consequence of the positive impact of magnesium in wheat germ. The daily consumption of 45 g wheat supplement for breakfast, 40 g between the meals, and 40 g for supper reduces 75% of fatigue after two months (45), which accords with our study. Chou et al. have reported the positive impact of vitamin B group on reducing the severity of anxiety, mood swings, fatigue, swelling, palpitation, headache, appetite change, depression, frequent crying, forgetfulness, sleep disorders and tension, which is in line with the present research, and might be due to the effect of the vitamin B in wheat germ (46).
The number of consumed painkillers remarkably reduced after intervention in the wheat germ extract group (p<0.001); while there was not a statistically significant difference in the placebo group on this score. The triple-blind clinical trial revealed that using 100 units of vitamin E per 6 hours for three days decreases the number of consumed painkillers (47), which is in line with our findings, perhaps because of the effect of the vitamin E of wheat germ.
Our study did not show a significant difference between the two groups with respect to the side-effects; in fact, it is not possible to attribute any specific side-effect to wheat germ in this study. The findings of this research prove the effectiveness and safety of the daily intake of 1200 mg wheat germ extract for reducing PMS symptoms.
Padalia et al., in their review article, titled “Examining the Property of Wheat Germ Extract”, reported no side-effects in various studies conducted in this field (48), which is in accordance with our study. The examination of the effect of wheat germ extract on autoimmune diseases and cancers did not reveal any side-effects, either (49). On base of document side effects of herbal products are less than synthetic drugs (50).
Conclusion
Consumption of wheat germ extract mitigated the severity of the general, physical, and psychological symptoms, which was observed after the first month of treatment. The therapeutic effects of wheat germ extract can be justified in view of its compounds such as vitamins B6 and E, calcium, zinc, linoleic acid, and magnesium whose effectiveness has been proved in previous studies. Wheat germ extract does not exert any effect on menstrual bleeding patterns; also, no particular side-effects were observed, and it seems that wheat germ extract can be used for controlling the premenstrual syndrome symptoms.
References
-
1.
Canning S, Waterman M, Orsi N, Ayres J, Simpson N, Dye L. The efficacy of Hypericum perforatum (St John’s Wort) for the treatment of premenstrual syndrome. CNS Drugs. 2010;24:207-225. [PubMed ID: 20155996].
-
2.
Georgantopoulou C, Field S. Treatment of premenstrual syndrome with the desogestrel-only pill (Cerazette) in an adolescent girl. J. Pediatr. Adolesc. Gynecol. 2009;22:1-3.
-
3.
Thys-Jacobs S. Micronutrient supplement with calcium, vitamin D or Calcium and vitamin D combination for premenstrual syndrome, postpartum depression, depression and panic attacks. J. Am. College of Nutr. 2012;19:220-227.
-
4.
Chocano-Bedoya PO. Micronutrient Intake and Premenstrual Syndrome [Dissertation]. Amherst: University of Massachusetts; 2011. p. 300-302.
-
5.
Marrif HI. Premenstrual syndrome, pharmaceuticals and alternative management. Eur. J. Soc. Sci. 2011;21:216.
-
6.
Hoyer J, Burmann I, Kieseler M-L, Vollrath F, Hellrung L, Arelin K, Roggenhofer E, Villringer A, Sacher J. Menstrual Cycle phase modulates emotional conflict processing in women with and without premenstrual syndrome (PMS)–a pilot study. J.PloS One. 2013;8:1-8.
-
7.
Seedhom AE, Mohammed ES, Mahfouz EM. Life style factors associated with premenstrual syndrome among El-Minia University Students, Egypt. ISRN Public Health. 2013;2013:1-6.
-
8.
Delaram M, Jafari F. The effect of fennel on the premenstrual syndrome. Knowledge Health. 2011;6:1-6.
-
9.
Sadler C, Smith H, Hammond J, Bayly R, Borland S, Panay N, Crook D, Inskip H. Lifestyle factors, hormonal contraception, and premenstrual symptoms: the United Kingdom Southampton Women's Survey. J. Women's Health. 2010;19:391-396.
-
10.
Joshi J, Pandey S, Galvankar P, Gogate J. Prevalence of premenstrual symptoms: Preliminary analysis and brief review of management strategies. J. Mid-life Health. 2010;1:30-34.
-
11.
Cunningham J, Yonkers KA, O'Brien S, Eriksson E. Update on research and treatment of premenstrual dysphoric disorder. J.Harvard Rev. Psychiatr. 2009;17:120-137.
-
12.
Biggs WS, Demuth RH. Premenstrual syndrome and premenstrual dysphoric disorder. J. Am. Fam. Phys. 2011;84:918-924.
-
13.
Golmakani N, Zagami SE. Use of medicinal plants in the treatment of premenstrual syndrome: a review. J. Am. Sci. 2011;7:60-64.
-
14.
Fritz A, LeonSperoff M. Clinical Gynecologic Endocrinology and Infertility. 8th ed. Philadelphia: Lippincott Williams and Wilkins; 2011. p. 1-1439.
-
15.
Wakil L, Meltzer-Brody S, Girdler S. Premenstrual dysphoric disorder: How to alleviate her suffering. J. Obstetrics Gynecol. Int. 2012;22:11-22.
-
16.
Jurgens T, Whelan AM. Advising patients on the use of natural health products to treat premenstrual syndrome. J. Canad. Pharm. 2009;142:228-233.
-
17.
Yonkers KA, O'Brien P, Eriksson E. Premenstrual syndrome. Lancet. 2008;371:1200-1210. [PubMed ID: 18395582].
-
18.
Shulman LP. Gynecological management of premenstrual symptoms. Curr. Pain Headache Rep. 2010;14:367-375. [PubMed ID: 20665250].
-
19.
Shooshtari MH, Hosseinpoor S, Tirandaz F, Mirhosseini M. Prevalence of premenstrual dysphoric disorder in female students of medical sciences. ISRN Public HealthAmerican Based Res. J. 2013;2:19-24.
-
20.
Kumar P, Yadava R, Gollen B, Kumar S, Verma R, Yadav S. Nutritional contents and medicinal properties of wheat: a review. J. Life Sci. Med. Res. 2011;22:1-10.
-
21.
Potter J, Bouyer J, Trussell J, Moreau C. Premenstrual syndrome prevalence and fluctuation over time: results from a French population-based survey. J. Women's Health. 2009;18:31-39.
-
22.
Zukov I, Ptácek R, Raboch J, Domluvilová D, Kuzelová H, Fischer S, Kozelek P. Premenstrual dysphoric disorder-review of actual findings about mental disorders related to menstrual cycle and possibilities of their therapy. J. Prague Med. Rep. 2010;111:12-24.
-
23.
Sabet-Birjandi S, Tadayon-Najafabadi M, Siyahpooshan A, Haghighizadeh M. Comparision the effect of Hypericum perforatum and vitamin B6 in the treatment of premenstrual syndrome. J. Zahedan Res. Med. Sci. 2011;13:1-5.
-
24.
Mirabi P, Alamolhoda SH, Esmaeilzadeh S, Mojab F. Effect of medicinal herbs on primary dysmenorrhoea-a systematic review. Iran. J. Pharm. Res. 2014;13:757-767. [PubMed ID: 25276177].
-
25.
Namazi M, Amir Ali Akbari S, Mojab F, Talebi A, Alavi Majd H, Jannesari S. Effects of Citrus aurantium (bitter orange) on the Severity of First-Stage Labor Pain. Iran. J. Pharm. Res. 2014;13:1011-1018. [PubMed ID: 25276203].
-
26.
Desai TR. Investigation intothe mechanism of action and effects of Triticum aestivum (wheat) grass [Dissertation]. India: Saurashtra University; 2005. p. 1-183.
-
27.
Singh N, Verma P, Pandey B. Therapeutic potential of organic Triticum aestivum Linn (wheat grass) in prevention and treatment of chronic diseases: an overview. J. Int. Pharm. Sci. Drug Res. 2012;4:10-14.
-
28.
Shah KV, Kapupara PK, Desai TR. Determination of sodium, potassium, calcium and lithium in a wheat grass by flame photometry. J. Int. Pharm. Sci. 2011;3:180-188.
-
29.
Panay N. Management of premenstrual syndrome. J. Fam. Planning Reprod. Health Care. 2009;35:187-194.
-
30.
Allais G, Castagnoli Gabellari I, Burzio C, Rolando S, De Lorenzo C, Mana O, Benedetto C. Premenstrual syndrome and migraine. J. Neurol. Sci. 2012;33:111-115.
-
31.
Zaka M, Mahmood KT. Premenstrual syndrome-a review. J. Pharm. Sci. Res. 2012;4:1684-1691.
-
32.
Ghanbari Z, Haghollahi F, Shariat M, Foroshani AR, Ashrafi M. Effects of calcium supplement therapy in women with premenstrual syndrome. Taiwanese J. Obstetrics Gynecol. 2009;48:124-129.
-
33.
Pourmohsen M, Zounemat KA, Taavoni S, Hosseini AF. Effect of combined calcium and vitamin E consumption on premenstrual syndrome. J. Iran Nursing Tehran Univ. Med. Sci. Health Services. 2010;23:8-14.
-
34.
Alexander L. Natural Wellness Strategies for the Menstrual Years. Scotland: Findhorn Press; 2013. p. 1-158.
-
35.
Chocano-Bedoya PO, Manson JE, Hankinson SE, Willett WC, Johnson SR, Chasan-Taber L, Ronnenberg AG, Bigelow C, Bertone-Johnson ER. Dietary B vitamin intake and incident premenstrual syndrome. J. Am. Clin. Nutr. 2011;93:1080-1086.
-
36.
Ozgoli G, Shahveh M, Esmaielli S, Nassiri N. Essential oil of Citrus sinensis for the treatment of premenstrual syndrome; a randomized double blind placebo controlled trial. J. Reprod. Infertil. 2011;2:123-129.
-
37.
Suija K, Rajala U, Jokelainen J, Liukkonen T, Härkönen P, Keinänen-Kiukaanniemi S, Timonen M. Validation of the Whooley questions and the Beck Depression Inventory in older adults. J. Scand. J. Primary Health Care. 2012;30:259-264.
-
38.
Seddigh R, Shariat SV, Gharraee B, Azarnik S. Evaluation of relationship between severity of depression and chess skill in members with international rating scale in Chess Federation of Islamic Republic of Iran in 1389. J. Razi Med. Sci. 2012;19:29-36.
-
39.
Salehi L, Salehi F. Effect of vitamin B6 on premenstrual syndrome. Sci. J. Kurdistan Univ. Med. Sci. 2007;12:42.
-
40.
Kwan I, Onwude JL. Premenstrual syndrome. J.Clin. Evidence. 2009;12:56-88.
-
41.
Braverman PK. Premenstrual syndrome and premenstrual dysphoric disorder. J. Pediatr. Adolesc. Gynecol. 2007;20:3-12. [PubMed ID: 17289510].
-
42.
Merete C, Falcon LM, Tucker KL. Vitamin B6 is associated with depressive symptomatology in Massachusetts elders. J. Am. College Nutr. 2008;27:421-427.
-
43.
Rapkin AJ, Mikacich JA. Premenstrual syndrome and premenstrual dysphoric disorder in adolescents. Curr. Opn.Obstetrics Gynecol. 2008;20:455-463.
-
44.
Sutariya S, Talsania N, Shah C, Patel M. An interventional study (calcium supplementation and health education) on premenstrual syndrome-effect on premenstrual and menstrual symptoms. National J.Commun. Med. 2011;2:100-104.
-
45.
Kochhar A, Gulati K, Sachdeva R. Impact of instant wheat meal supplementation and nutrition counselling on anthropometry and blood pressure of non insulin dependent diabetics. Ethno-Med. 2009;3:69-74.
-
46.
Chou PB, Morse CA, Xu H. A controlled trial of Chinese herbal medicine for premenstrual syndrome. J. Psychosomatic Obstetrics & Gynecol. 2008;29:189-196.
-
47.
Moslemi L, Bekhradi R, Moghaddam GT, Tabari GM. Comparative effect of fennel extract on the intensity of primary dysmenorrhea. J. Afr. Pharm. Pharmacol. 2012;6:1770-1773.
-
48.
Padalia S, Drabu S, Raheja I, Gupta A, Dhamija M. Multitude potential of wheatgrass juice (Green Blood): An overview. Chronicles of YoungScientists. 2010;1:23-28.
-
49.
Boros LG, Nichelatti M, Shoenfeld Y. Fermented wheat germ extract (Avemar) in the treatment of cancer and autoimmune diseases. J.Ann. NY Acad. Sci. 2005;1051:529-542.
-
50.
Younesy S, Amir Ali Akbari S, Esmaeili S, Alavimajd H, Nouraei S. Effects of fenugreek seed on the severity and systemic symptoms of dysmenorrhea. J. Reprod. Infertil. 2014;15:41-48. [PubMed ID: 24695380].