Tissue Distribution of 125I-human Nonspecific Polyclonal IgG in Normal and Induced Inflammation Mice

Soraya Shahhosseini; Mohammad Hossein Babaei; Reza Najafi

doi:10.22037/ijpr.2010.11

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.11

Tissue Distribution of 125I-human Nonspecific Polyclonal IgG in Normal and Induced Inflammation Mice

Author(s):

Soraya Shahhosseini^1,*,

Mohammad Hossein Babaei²,

Reza Najafi²

1a Department of Pharmacetical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services, Tehran, Iran

2Radioisotopes Division, Nuclear Research Center, Atomic Energy Organization of Iran, Tehran, Iran

IJ Pharmaceutical Research:Vol. 1, issue 1; 55-59

Published online:Nov 20, 2010

Article type:Research Article

Received:Apr 01, 2002

Accepted:Jul 01, 2002

How to Cite:Soraya ShahhosseiniMohammad Hossein BabaeiReza NajafiTissue Distribution of 125I-human Nonspecific Polyclonal IgG in Normal and Induced Inflammation Mice.Iran J Pharm Res.1(1):e127587.https://doi.org/10.22037/ijpr.2010.11.

Abstract

Many different radiolabeled antibodies have been used for radioimmunotherapy and radioimmunoscintigraphy of human diseases in animal experiments. In order to study the in vivo tissue distribution of antibody, we labeled human nonspecific polyclonal IgG with Na125I using chloramine-T method. An animal model was developed by injecting turpentine in the posterior left thigh of Balb/c mice. Tissue distribution of 125I-IgG was assessed in normal and induced inflammation mice. Although labeled IgG accumulated in normal tissues such as liver, spleen and blood, its localization in inflammatory left thigh was significantly different from normal right thigh. Thus, by using the radiolabeling methods of IgG in order to increase the target-to-background ratio, antibody scintigraphy could be suggested as a powerful diagnostic tool for inflammation.

Keywords

Percent Injected Dose per gram tissue (% ID/g)

Immunoglobulin (Ig)

Turpentine

Immunoscintigraphy

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Author(s):

Soraya Shahhosseini:[PubMed][Scholar]
Mohammad Hossein Babaei:[PubMed][Scholar]
Reza Najafi:[PubMed][Scholar]