Interaction of Cisplatin with Cellular Macromolecules: A Fourier Transform Infrared Spectroscopy Study

authors:

avatar Farshad H.Shirazi 1 , * , avatar Patrick T.T. Wong 2 , avatar Rakesh Goel 3

Dept. of Toxicology and Pharmacology, School of Pharmacy, Shaheed Beheshti Univerity of Medical Sciences, Tehran, Iran
Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, Canada
Ottawa Regional Cancer Centre, Ottawa, Canada
Iranian Journal of Pharmaceutical Research: Vol.2, issue 1; 11-15
published online: November 20, 2010
article type: Research Article
received: April 01, 2002
accepted: February 01, 2003
DOI: https://doi.org/10.22037/ijpr.2010.28

how to cite: H.Shirazi F, Wong P T, Goel R. Interaction of Cisplatin with Cellular Macromolecules: A Fourier Transform Infrared Spectroscopy Study. Iran J Pharm Res. 2003;2(1):e127602. https://doi.org/10.22037/ijpr.2010.28.

Abstract

Platinum is a metallic element, which may react with our cellular component through its involvement in cancer chemotherapy medications. Cisplatin is one of the most useful antineoplastic drugs against human ovarian carcinoma, which has the central element of platinum in its structure. The nature of chemical interaction between platinum and cellular macromolecules is yet to be understood. We examined the interactions of platinum with human ovarian OV 2008 cancer cells by infrared spectroscopy, through the exposure of this cell line to cisplatin. These studies showed that there was an interaction between cisplatin and DNA and proteins. Nature of these interactions and their possible effects are discussed in this paper. In summary, it has caused denaturation of proteins and modification of the interchain packing of the DNA. Our results show that infrared spectroscopy is a potentially useful technique for monitoring the interaction of elements and drugs with cell components.