In Vitro Antibacterial Activity of Rheum ribes Extract Obtained from Various Plant Parts Against Clinical Isolates of Gram-Negative Pathogens

authors:

avatar Bibi Sedigheh Fazly Bazzaz 1 , * , avatar Mehrangiz Khajehkaramadin 2 , avatar Hamid Reza Shokooheizadeh 2

School of Pharmacy and Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
Iranian Journal of Pharmaceutical Research: Vol.4, issue 2; 87-91
published online: November 20, 2010
article type: Research Article
received: April 20, 2004
accepted: October 20, 2004
DOI: https://doi.org/10.22037/ijpr.2010.621

how to cite: Fazly Bazzaz B S, Khajehkaramadin M, Shokooheizadeh H R. In Vitro Antibacterial Activity of Rheum ribes Extract Obtained from Various Plant Parts Against Clinical Isolates of Gram-Negative Pathogens. Iran J Pharm Res. 2005;4(2):e128230. https://doi.org/10.22037/ijpr.2010.621.

Abstract

Rheum ribes is among the Polygonaceae family which is endemic in Iran and a few neighboring countries. In this investigation, antibacterial effects of root, stalk and leaves extracts of Rheum ribes on a few common clinical isolates of gram negative pathogens were studied, using the cup plate and paper disc methods. Gram negative microorganisms studied were Escherichia coli, Klebsiella pneumoniae, Proteus spp., Pseudomonas aeruginosa and Neisseria gonorrhoeae.

For examination of pathogenic microorganisms in the cup plate and disc diffusion method, methanolic extracts of different plant parts at concentrations of 250 and 500 micrograms per cup or disc, were used.

The root and leaves extracts have demonstrated significant antibacterial activities in both methods. However, stalk extracts showed a lower degree of antibacterial activity than the root and leaves extracts. Generally, the extracts showed a broad spectrum of activity, although they were more effective against P. aeruginosa and Proteus spp. in comparison with the positive control. The results suggested that extracts of Rheum ribes could be effectively used against clinical isolates.