Production of Rosmarinic Acid in Echium amoenum Fisch. and C.A. Mey. Cell Cultures

Mitra Mehrabani; Mohammadreza Shams-Ardakani; Alireza Ghannadi; Nasrolah Ghassemi Dehkordi; Seyyed Ebrahim Sajjadi Jazi

doi:10.22037/ijpr.2010.625

IJ Pharmaceutical Research

Current Issue All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Contact Us Support

APC

Authors Guide Submit Manuscript

https://doi.org/10.22037/ijpr.2010.625

Production of Rosmarinic Acid in Echium amoenum Fisch. and C.A. Mey. Cell Cultures

Author(s):

Mitra Mehrabani^1,*,

Mohammadreza Shams-Ardakani²,

Alireza Ghannadi³,

Nasrolah Ghassemi Dehkordi³,

Seyyed Ebrahim Sajjadi Jazi³

1Department of Pharmacognosy, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

2Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

IJ Pharmaceutical Research:Vol. 4, issue 2; 111-115

Published online:Nov 20, 2010

Article type:Research Article

Received:Oct 20, 2004

Accepted:Apr 20, 2005

How to Cite:Mitra MehrabaniMohammadreza Shams-ArdakaniAlireza GhannadiNasrolah Ghassemi DehkordiSeyyed Ebrahim Sajjadi JaziProduction of Rosmarinic Acid in Echium amoenum Fisch. and C.A. Mey. Cell Cultures.Iran J Pharm Res.4(2):e128234.https://doi.org/10.22037/ijpr.2010.625.

Abstract

Echium amoenum Fisch. and C.A. Mey. (Boraginaceae) is a very popular medicinal plant which is used as a tonic, tranquillizer, diaphoretic, cough remedy, sore throat and pneumonia in Iran’s traditional medicine. Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, callus culture of E. amoenum and its major secondary metabolite were investigated. The callus culture of E. amoenum was initiated and established from seeds in MS media with three different ratios of plant growth regulatories: kinetin, 2,4-D and NAA. Methanolic extracts of freeze-dried calluses were compared by TLC and HPLC. The major secondary metabolite was separated by preparative HPLC and the structure of this pure compound was elucidated by UV, IR, one and two dimensional ¹H and ¹³C-NMR and Mass spectroscopy. Rosmarinic acid was identified by various spectroscopic methods from callus culture of E. amoenum. Rosmarinic acid is widespread within the plant cell tissue culture of the Lamiaceae and Boraginaceae families, although in insignificant quantities. Rosmarinic acid has an antimicrobial, antiviral, and anti-inflammatory effect, which makes it a valuable product for the pharmaceutical and cosmetic industries.

Keywords

Rosmarinic acid

Callus culture

Echium amoenum

comments

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Metrics

Get Permission (article level)

Purchasing Reprints

Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here ( https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.

Search Relations

Author(s):

Mitra Mehrabani:[PubMed][Scholar]
Mohammadreza Shams-Ardakani:[PubMed][Scholar]
Alireza Ghannadi:[PubMed][Scholar]
Nasrolah Ghassemi Dehkordi:[PubMed][Scholar]
Seyyed Ebrahim Sajjadi Jazi:[PubMed][Scholar]