Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma
Authors
Abstract
A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150×4 mm) and a UV detector (λ= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phase. The proteins were precipitated with an acetonitrile solution containing diltiazem as internal standard and the average recovery was 93.9±3.4%. The detection limit for risperidone in plasma was 0.5 ngml-1. The calibration curve was linear over the concentration range 2-50 ngml-1. The inter-day and intra-day assay coefficients of variation were found to be less than 5%. The present validated method was successfully used for pharmacokinetic studies of risperidone in human subjects.
Highlights
Copyright
© 2022, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
High-Performance Liquid Chromatographic Determination of Rivastigmine in Human Plasma for Application in Pharmacokinetic Studies
Amini H, Ahmadiani A. High-Performance Liquid Chromatographic Determination of Rivastigmine in Human Plasma for Application in Pharmacokinetic Studies. Iran J Pharm Res. 2010;9(2):e126070. doi: https://doi.org/10.22037/ijpr.2010.845
Reversed Phase High Performance Liquid Chromatographic Method for Determination of Quetiapine Fumarate in Pharmaceutical Formulation and in Spiked Human Urine
Vinay KB, Revanasiddappa HD, Rajendraprasad N, Ramesh PJ, Basavaiah K. Reversed Phase High Performance Liquid Chromatographic Method for Determination of Quetiapine Fumarate in Pharmaceutical Formulation and in Spiked Human Urine. J Rep Pharm Sci. 2013;2(2):e147746. doi:
Simple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
Amini M, Khanavi M, Shafiee A. Simple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma. Iran J Pharm Res. 2022;3(2):e128189. doi: https://doi.org/10.22037/ijpr.2010.583
A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin
Sobhani H, Shafaati A, Nafissi-Varcheh N, Aboofazeli R. A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin. Iran J Pharm Res. 2013;12(Suppl):e125628. doi: https://doi.org/10.22037/ijpr.2013.1274
Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection
Dasht Bozorg B, Goodarzi A, Fahimi F, Tabarsi P, Shahsavari N, et al. Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection. Iran J Pharm Res. 2019;18(4):e124712. doi: https://doi.org/10.22037/ijpr.2019.1100849
- Scopus by DOI: 9
Last Update: 3 weeks ago
- Scopus by Title: 9
Last Update: 3 weeks ago
- Scopus by Title (Ref): 0
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 6 days ago
- OpenAlex: 0
Last Update: 5 hours ago
- OpenCitations: 1
Last Update: 4 days ago