Isolation and Identification of Calystegines in Root Cultures of four Physalis Species

authors:

avatar Mohammad Ebrahim Azemi 1 , avatar Mahmoud Mosaddegh 2 , * , avatar Abdol Majid Cheraghali 3 , avatar Forough Namjooyan 1 , avatar Birgit Dräger 4

Pharmacognosy Department, Faculty of Pharmacy, Shaheed Beheshti Medical University of Science, Tehran, Iran
Traditional Medicine and Materia Medica Reacearch Center, Tehran, Iran
Pharmacology Department, Baghyyatallah Medical University of Science, Tehran, Iran
Institute of Pharmaceutical Biology, Faculty of Pharmacy, Martin-Luther- University, Halle-Wittenberg, Germany
Iranian Journal of Pharmaceutical Research: Vol.5, issue 1; 69-72
published online: November 20, 2010
article type: Research Article
received: January 21, 2006
accepted: May 21, 2006
DOI: https://doi.org/10.22037/ijpr.2010.656

how to cite: Azemi M E, Mosaddegh M, Cheraghali A M, Namjooyan F, Dräger B. Isolation and Identification of Calystegines in Root Cultures of four Physalis Species. Iran J Pharm Res. 2006;5(1):e128266. https://doi.org/10.22037/ijpr.2010.656.

Abstract

Calystegines were isolated from the root cultures of Physalis divaricata, P. pubescens, P. philadelphia,and P. philadelphia (solanaceae). Calystegines of P. divaricata were identified as calystegine A3, A5, B1 and B2, with concentrations of 6.99, 4.41, 8.52, and 14.7 µg/g in fresh mass, respectively. Root cultures of P.pubescens contain calystegine A3, B1 and B2, P. philadelphia only contains calystegine B2 and P. philadelphia root cultures solely consist of calystegine A3. Isolation and identification of calystegines have been achieved by ion exchange column chromatography and gas chromatography, using authentic samples.