The Pharmacological Effects of Odonthobuthus doriae Scorpion Venom and Its Extracted Fractions on Neuro-Muscular Transmission

Amir Jalali; Zahra Hossininasab; Zynab Ehsani; Amir-Abbas Zare; Afshin Zarghi; Hossein Vatanpour

doi:10.22037/ijpr.2010.661

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.661

The Pharmacological Effects of Odonthobuthus doriae Scorpion Venom and Its Extracted Fractions on Neuro-Muscular Transmission

Author(s):

Amir Jalali¹,

Zahra Hossininasab²,

Zynab Ehsani²,

Amir-Abbas Zare³,

Afshin Zarghi⁴,

Hossein Vatanpour^2,*

1Dept. of Toxicology and Pharmacology, Faculty of Pharmacy, Jundishapour University of Medical Sciences, Ahwaz, Iran

2Dept. of Toxicology and Pharmacology, Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

3Razi Vaccine and Serum Research Institute, Karaj, Iran

4Dept. of Pharmaceutical Chemistry, Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

IJ Pharmaceutical Research:Vol. 5, issue 2; 101-105

Published online:Nov 20, 2010

Article type:Research Article

Received:Jan 21, 2005

Accepted:Oct 21, 2005

How to Cite:Amir JalaliZahra HossininasabZynab EhsaniAmir-Abbas ZareAfshin ZarghiHossein Vatanpouret al.The Pharmacological Effects of Odonthobuthus doriae Scorpion Venom and Its Extracted Fractions on Neuro-Muscular Transmission.Iran J Pharm Res.5(2):e128271.https://doi.org/10.22037/ijpr.2010.661.

Abstract

The effect of Odonthobuthos doriae (O.d) scorpion venom at 0/3, 1 and 3, 10 µg/ml concentrations were investigated on nerve-muscle transmission, using the Twitch tension technique. A concentration of 0.3 µg/ml caused a small change in the twitch height in response to indirect muscle stimulation, but higher concentrations (1, 3, 10 µg/ml) caused a transient augmentation in twitch response followed by a large contracture in the chick biventer cervices (CBC) preparation. This effect could be defined as a complex action of the venom, predominately presynaptic, in which its’ effects on postjunctional synapses is also maintained.

In order to find out which bioactive fraction could explain the venom effects, the soluble crude venom was partially separated by the gel filtration method, using a Sephadex G₅₀ column, and four fractions were separated. Two of the four purified fractions (O.d F₃, O.d F₄) were characterized as toxic and their LD₅₀ values were lower than the crude venom. Unlike the O.d F₁ and O.d F₂ fractions, O.d F₃ and O.d F₄ fractions caused a significant block in the twitch and contracture, in comparison to the control sample . in conclusion, fractions O.d F₃ and O.d F₄ are supposed to be as the biological active components of the O.d. venom.

Keywords

Scorpion venom

Odonthobuthus doriae

Pharmacological responses

Neuro-muscular

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Author(s):

Amir Jalali:[PubMed][Scholar]
Zahra Hossininasab:[PubMed][Scholar]
Zynab Ehsani:[PubMed][Scholar]
Amir-Abbas Zare:[PubMed][Scholar]
Afshin Zarghi:[PubMed][Scholar]
Hossein Vatanpour:[PubMed][Scholar]