Evaluation of Neutralizing Efficacy and Possible Microbial Cell Toxicity of a Universal Neutralizer Proposed by the CTPA

Hadi Mehrgan; Ferial Elmi; Mohammad Reza Fazeli; Ahmad Reza Shahverdi; Nasrin Samadi

doi:10.22037/ijpr.2010.672

IJ Pharmaceutical Research

Current Issue All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Contact Us Support

APC

Authors Guide Submit Manuscript

https://doi.org/10.22037/ijpr.2010.672

Evaluation of Neutralizing Efficacy and Possible Microbial Cell Toxicity of a Universal Neutralizer Proposed by the CTPA

Author(s):

Hadi Mehrgan^1,*,

Ferial Elmi²,

Mohammad Reza Fazeli²,

Ahmad Reza Shahverdi³,

Nasrin Samadi²

1Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

2Department of Drug and Food Control, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3Department of Biotechnology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

IJ Pharmaceutical Research:Vol. 5, issue 3; 173-178

Published online:Nov 20, 2010

Article type:Research Article

Received:Jan 21, 2006

Accepted:May 21, 2006

How to Cite:Hadi MehrganFerial ElmiMohammad Reza FazeliAhmad Reza ShahverdiNasrin SamadiEvaluation of Neutralizing Efficacy and Possible Microbial Cell Toxicity of a Universal Neutralizer Proposed by the CTPA.Iran J Pharm Res.5(3):e128282.https://doi.org/10.22037/ijpr.2010.672.

Abstract

The purpose of this research was to study the neutralization efficacy of a universal neutralizer proposed by the CTPA on a range of antimicrobial agents and its potential toxicity for microorganisms that are used for antimicrobial preservation testing. Several types of antimicrobial agents including a mixture of methylisothiazolinone and its 5-chloro derivative, dimethylol dimethyl hydantoin, Quaternium-15^®, Bronopol^®, benzalkonium chloride, phenoxyethanol, methyl and propyl paraben, chlorhexidine, imidazolidinyl urea, triclosan and thiomersal built-in pharmaceutical, cosmetic or health care products, as well as 6 types of these antimicrobials, i.e. a combination of methyl paraben (0.18% w/v) and propyl paraben (0.02%), imidazolidinyl urea (0.3%), chlorhexidine (0.01%), benzalkonium chloride (0.02%), Bronopol^® (0.1%) and thiomersal (0.02%) in their pure and soluble form were tested. Validation of microbial recovery was carried out according to the US Pharmacopeia 27 guidelines. The universal neutralizer could inactivate all the studied antimicrobial agents against different strains tested except Staphylococcus aureus. With regards to this microorganism, only seven preservative chemicals consisting of parabens, dimethylol dimethyl hydantoin, Quaternium-15^®, Bronopol^®, benzalkonium chloride, Phenonip^® and imidazolidinyl urea were effectively inactivated. In addition, a pure solution of 0.02% thiomersal retained its antimicrobial properties against all studied microorganisms. The neutralizer solution showed no toxicity on any of the test organisms. In conclusion, CTPA proposed neutralizing solution is not an inclusive neutralizer. Moreover, each organism to be used in the test must be included in the validation study.

Keywords

Preservative

Antimicrobial agent

Neutralization

Validation of microbial recovery

comments

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Metrics

Get Permission (article level)

Purchasing Reprints

Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here ( https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.

Search Relations

Author(s):

Hadi Mehrgan:[PubMed][Scholar]
Ferial Elmi:[PubMed][Scholar]
Mohammad Reza Fazeli:[PubMed][Scholar]
Ahmad Reza Shahverdi:[PubMed][Scholar]
Nasrin Samadi:[PubMed][Scholar]