Lovastatin Incubation Improves Acetylcholine-Induced Relaxation in Isolated Aortic Rings of Diabetic Rat

Heydar Parsaee; Mohsen Imenshahidi; Zahra Fatehi

doi:10.22037/ijpr.2010.674

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.674

Lovastatin Incubation Improves Acetylcholine-Induced Relaxation in Isolated Aortic Rings of Diabetic Rat

Author(s):

Heydar Parsaee^1,*,

Mohsen Imenshahidi¹,

Zahra Fatehi¹

1Department of Pharmacology, Department of Physiology, Pharmacological Research Center of Medicinal Plants Medical School, Mashhad University of Medical Sciences, Mashhad, Iran

IJ Pharmaceutical Research:Vol. 5, issue 3; 191-198

Published online:Nov 20, 2010

Article type:Research Article

Received:May 21, 2005

Accepted:Nov 21, 2005

How to Cite:Heydar ParsaeeMohsen ImenshahidiZahra FatehiLovastatin Incubation Improves Acetylcholine-Induced Relaxation in Isolated Aortic Rings of Diabetic Rat.Iran J Pharm Res.5(3):e128284.https://doi.org/10.22037/ijpr.2010.674.

Abstract

To evaluate the acute effect of lovastatin on diabetic endothelial dysfunction, we examined this effect on the aortic rings of streptozotocin-diabetic rats. The endothelial function was assessed in aortic rings isolated from diabetic rats, 12 weeks after treatment with streptozotocin (45 mg/kg, i.p.). The concentration-response curve to acetylcholine (Ach) in the aortic rings precontracted with phenylephrine (10^-6 M) was significantly diminished in diabetic groups; and maximum relaxation in control and diabetic groups were 82±1.93% and 48±2.39% respectively (a 42% decrease, P<0.001). Incubation with lovastatin (10^-5M) for 10 min, significantly improved the Ach-induced relaxation of diabetic groups and the maximum relaxation increased to 74.2 ±3.3% (a 54% increase, P<0.001).

Incubation with NG –nitro-L-arginine methyl ester hydrochloride (L-NAME; 5×10^-7 M) for 20 min eliminated a significant difference in Ach – induced relaxation responses in diabetic and control groups and also eliminated the improving effect of lovastatin in diabetic groups. On the other hand 10 min incubation with indomethacin (10^-5 M) did not eliminate the difference in Ach-induced relaxation responses in diabetic and control groups and also did not eliminate the improving effect of lovastatin in diabetic groups. Lovastatin did not modify sodium nitroprosside-induced relaxation in either diabetic or control groups and also did not induce any direct relaxation.

Therefore, it is concluded that incubation of aortic rings with lovastatin significantly improves endothelium-dependent relaxation in diabetic groups by increasing the nitric oxide bioavailability, most probably due to its’ antioxidant effects.

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Author(s):

Heydar Parsaee:[PubMed][Scholar]
Mohsen Imenshahidi:[PubMed][Scholar]
Zahra Fatehi:[PubMed][Scholar]