Impact of Duration and Severity of Persistent Pain on Programmed Cell Death
Authors
Abstract
Programmed cell death is a highly regulated form of cell death, mostly distinguished by the activation of a family of cystein-aspartate proteases (caspases) that cleave various proteins resulting in morphological and biochemical changes characteristic of this form of cell death. Several recent studies have addressed the role of programmed cell death in inflammatory and chronic pain states. Caspase-3 plays a central role in mediating nuclear programmed cell death including chromatin condensation and DNA fragmentation as well as cell blebbing. The aims of this study were to investigate the effect of duration and severity of persistent pain on the induction of programmed cell death. Formalin was administered subcutaneously in the Wistar rat hind paws for 1, 4 or 7 consecutive days, and then the activity of caspase-3 was measured in both the rat liver and brain cells. Morphological changes characterizing programmed cell death were also studied using the Sigma’s Apoptosis Detection kit, Annexin V-Cy3. Our findings showed that caspase-3 activity and apoptotic phenotype significantly increased in liver but not brain cells following the increase in duration and severity of formalin induced persistent pain.
Copyright
© 2022, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Evaluation of Molecular and Cellular Alterations Induced by Neuropathic Pain in Rat Brain Glial cells
Rezaei M, Karimian L, Shafaghi B, Noubarani M, Salecheh M, et al. Evaluation of Molecular and Cellular Alterations Induced by Neuropathic Pain in Rat Brain Glial cells. Iran J Pharm Res. 2021;20(1):e124471. doi: https://doi.org/10.22037/ijpr.2020.113052.14089
Inhibition of Different Pain Pathways Attenuates Oxidative Stress in Glial Cells: A Mechanistic View on Neuroprotective Effects of Different Types of Analgesics
Eskandari MR, Eftekhari P, Abbaszadeh S, Noubarani M, Shafaghi B, et al. Inhibition of Different Pain Pathways Attenuates Oxidative Stress in Glial Cells: A Mechanistic View on Neuroprotective Effects of Different Types of Analgesics. Iran J Pharm Res. 2021;20(3):e124293. doi: https://doi.org/10.22037/ijpr.2021.114476.14871
Microglia are involved in pain related behaviors during the acute and chronic phase of arthritis inflammation
Nasseri B, Nazemian V, Manaheji H, Zarringhalam J. Microglia are involved in pain related behaviors during the acute and chronic phase of arthritis inflammation. J Cell Mol Anesth. 2016;1(4):e149523. doi: https://doi.org/10.22037/jcma.v1i4.13557
The Effects of Chlorpyrifos on Hippocampal Caspase-3 Levels and Passive Avoidance Memory in Rats
Nikbin S, Jameie SB, Azarbayjani MA, Hosseini N. The Effects of Chlorpyrifos on Hippocampal Caspase-3 Levels and Passive Avoidance Memory in Rats. Thrita J Neu. 2022;11(1):e123356. doi: https://doi.org/10.5812/thrita-123356
Activation of Microglial Cells: the Bridge between the Immune System and Pain in Central Nervous System
Dabbagh A. Activation of Microglial Cells: the Bridge between the Immune System and Pain in Central Nervous System. J Cell Mol Anesth. 2016;1(4):e149526. doi: https://doi.org/10.22037/jcma.v1i4.13620
- Scopus by DOI: 3
Last Update: 1 month ago
- Scopus by Title: 3
Last Update: 1 month ago
- Scopus by Title (Ref): 0
Last Update: 1 month ago
- CrossRef: 0
Last Update: 4 hours ago