The Effect of α-Tocopherol on Copper Binding to Low Density Lipoprotein

How to Cite:Ghaffari MA, Saffari MR, Ghiasvand T. The Effect of α-Tocopherol on Copper Binding to Low Density Lipoprotein. Iran J Pharm Res. 2022;5(3):e128287. doi: https://doi.org/10.22037/ijpr.2010.677

Abstract

The oxidative modification of low density lipoprotein (LDL) may play an important role in atherogenesis. Antioxidants that can prevent LDL oxidation may act as antiatherogens. Our understanding of the mechanism of LDL oxidation and factors that determine its susceptibility to oxidation is still incomplete. Copper is a candidate for oxidizing LDL in atherosclerotic lesions. The binding of copper ions to LDL is usually thought to be a prerequisite for LDL oxidation by copper. Therefore we investigated the effect of α-tocopherol (as a major fat soluble antioxidant) on copper bound to LDL and furthermore effect of this binding on the susceptibility of LDL to oxidative modification.

In this study LDL was isolated from EDTA-plasma (1 mg EDTA/ml blood) by ultracentrifugation using a single-step discontinuous gradient. Then α-tocopherol was added to LDL and incubated for 1 h at 37° C. The oxidation rate of LDL was estimated by thiobarbitoric acid reactive substances (TBARS) after CuSO₄ addition. Finally, the effect of α-tocopherol on formation of LDL-copper complex by gel filtration was studied.

Our results showed that α-tocopherol (dose dependently) suppressed the formation of TBARS and LDL-copper complex. The α-tocopherol with concentrations of 10, 50 and 100 µM reduced susceptibility of LDL to oxidative modification approximately by 2, 13 and 21 percent, respectively. Furthermore, addition of α-tocopherol to the LDL and CuSO₄ mixture containing before hand prevented the formation of LDL-copper complex, approximately by 30 percent.

In conclusion we found that α-tocopherol with inhibition of copper binding to LDL may decrease the susceptibility of LDL oxidation to this ion and thus could play a role in prevention of atherosclerosis.

Keywords

α-Tocopherol

Low density lipoprotein (LDL)

oxidation

Atherosclerosis

copper

Import into EndNote Import into BibTex

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 3
Last Update: 1 week ago
Scopus by Title: 3
Last Update: 1 week ago
Scopus by Title (Ref): 0
Last Update: 1 week ago
CrossRef: 0
Last Update: 5 days ago

Metrics

Licensing and reuse information

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Mohammad Ali Ghaffari:[PubMed][Scholar]
Mohammad Reza Saffari:[PubMed][Scholar]
Taibeh Ghiasvand:[PubMed][Scholar]

Outlines

The Effect of α-Tocopherol on Copper Binding to Low Density Lipoprotein

Abstract

Crossmark

Checking

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 5 days ago