Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves

authors:

avatar Fatemeh Fathiazad 1 , * , avatar Abbas Delazar 1 , avatar Roya Amiri 1 , avatar Satyajit D. Sarker 2

School of Pharmacy and Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
Phytopharmaceutical Research Laboratory, School of Pharmacy, The Robert Gordon University, School Hill, Aberdeen Ab10 1FR, Scotland, UK
Iranian Journal of Pharmaceutical Research: Vol.5, issue 3; 222-227
published online: November 20, 2010
article type: Research Article
received: January 21, 2005
accepted: November 21, 2005
DOI: https://doi.org/10.22037/ijpr.2010.680

how to cite: Fathiazad F, Delazar A, Amiri R, Sarker S D. Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves. Iran J Pharm Res. 2006;5(3):e128290. https://doi.org/10.22037/ijpr.2010.680.

Abstract

The potential of tobacco waste as a viable source of medicinally important flavonoids like rutin has been investigated. Three flavonoids, apigenin, quercetin and rutin, have been isolated from waste tobacco leaves, and their identities have been confirmed by UV-visible, 1H-NMR and 13C-NMR spectroscopy. By using analytical HPLC, the amount of rutin present in the tobacco leaves, before and after fermentation, and also in waste tobacco leaves, has been determined as 1.5, 0.5 and 0.6%, respectively.