A Kinetic Comparison on the Inhibition of Adenosine Deaminase by Purine Drugs

Ghasem Ataie; Soghra Bagheri; Adeleh Divsalar; Ali Akbar Saboury; Shahrokh Safarian; Saeed Namaki; Ali Akbar Moosavi- Movahedi

doi:10.22037/ijpr.2010.697

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.697

A Kinetic Comparison on the Inhibition of Adenosine Deaminase by Purine Drugs

Author(s):

Ghasem Ataie^1,*,

Soghra Bagheri¹,

Adeleh Divsalar¹,

Ali Akbar Saboury¹,

Shahrokh Safarian²,

Saeed Namaki³,

Ali Akbar Moosavi- Movahedi¹

1Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

2Department of Biology, College of Science, University of Tehran, Tehran, Iran

3Faculty of Paramedical Science, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

IJ Pharmaceutical Research:Vol. 6, issue 1; 43-50

Published online:Nov 20, 2010

Article type:Research Article

Received:Jan 21, 2006

Accepted:Aug 21, 2006

How to Cite:Ghasem AtaieSoghra BagheriAdeleh DivsalarAli Akbar SabouryShahrokh SafarianSaeed NamakiAli Akbar Moosavi- Movahediet al.A Kinetic Comparison on the Inhibition of Adenosine Deaminase by Purine Drugs.Iran J Pharm Res.6(1):e128308.https://doi.org/10.22037/ijpr.2010.697.

Abstract

The effects of allopurinol, acyclovir and theophylline on the activity of adenosine deaminase (ADA) were studied in 50 mM sodium phosphate buffer pH 7.5 at 27°C, using a UV– Vis spectrophotometer. Adenosine deaminase is inhibited by these ligands, via different types of inhibition. Allopurinol, as a transition state analog of xanthine oxidase, and acyclovir competitively inhibit the catalytic activity of ADA. Inhibition constant values are 285 and 231 µM for allopurinol and acyclovir, respectively. Theophylline acts as a non-competitive inhibitor for ADA, which shows different affinity binding sites at various drug concentrations. There were two different types of inhibition constant, one of them due to a low concentration of the drug (K_i = 56 µM) and the other appearing at higher concentrations of theophylline (K_i = 201 µM). Thermodynamic parameters also show that ADA has two binding sites for theophylline.

The comparison of inhibition constant for inosine (K_i=143 µM) and acyclovir (K_i = 231 µM) elucidates the critical role of the ribose ring within the inosine structure, relative to the open ring of acyclovir. Comparison of the inhibition constant of theobromine (K_i= 311 µM) with inosine (K_i= 143 µM) shows the critical binding role of N⁷ position within the purine ring. Interestingly, the N⁷ position in allopurinol is replaced by a CH₂ group, which demonstrates the lower inhibiting potency of allopurinol (K_i = 285 µM) relative to inosine (K_i = 143 µM). In a structural sense, a comparison made between the structure of theophylline and theobromine besides a comparison between the inhibition constant of theophylline (K_i = 56 µM at low and 201 µM at higher concentrations) and caffeine (K_i = 342 µM) indicate that substitution of a bulky group in N¹ and N⁷ positions of purine has a critical role in the binding affinity of the above- mentioned inhibitors to the enzyme.

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Author(s):

Ghasem Ataie:[PubMed][Scholar]
Soghra Bagheri:[PubMed][Scholar]
Adeleh Divsalar:[PubMed][Scholar]
Ali Akbar Saboury:[PubMed][Scholar]
Shahrokh Safarian:[PubMed][Scholar]
Saeed Namaki:[PubMed][Scholar]
Ali Akbar Moosavi- Movahedi:[PubMed][Scholar]