Manganese and Iron Binding to Human Transferrin

How to Cite:Ali Asghar MoshtaghieAkbar BadiiMohammad FazilatiTaghi HassanzadehHadi KharaziManganese and Iron Binding to Human Transferrin.Iran J Pharm Res.6(2):e128317.https://doi.org/10.22037/ijpr.2010.706.

Abstract

The characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. Both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (Mn-tf) and 465 nm for iron-transferrin (Fe-tf). Addition of manganese (1.5 µg/ml) to the reaction mixture containing iron and apo-tf, reduced Fe binding to apo-tf by 20 percent, in comparison to the control sample. The binding of both metals to apo-tf appears to be time and pH dependent processes.

Using the equilibrium dialysis technique, the binding constant of manganese to apo-tf was also determined. The binding constatnt of Mn to apo-tf was calculated, using the Scatchard plot analysis. The calculated Ka was 3.1×10⁹ M^-1.

The binding of manganese and iron to human apo-tf has been discussed and compared in this work, using different biochemical techniques.

Keywords

comments

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

CrossRef 0

Metrics

Get Permission (article level)

Purchasing Reprints

Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here ( https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.

Search Relations

Author(s):

Ali Asghar Moshtaghie:[PubMed][Scholar]
Akbar Badii:[PubMed][Scholar]
Mohammad Fazilati:[PubMed][Scholar]
Taghi Hassanzadeh:[PubMed][Scholar]
Hadi Kharazi:[PubMed][Scholar]