Nasal Immunization by (PLGA) Nanospheres Encapsulated with Tetanus Toxoid and (CpG-ODN)

Mohsen Tafaghodi; Seyed Abolghasem Sajadi Tabassi; Mahmoud Reza Jaafari

doi:10.22037/ijpr.2010.715

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.715

Nasal Immunization by (PLGA) Nanospheres Encapsulated with Tetanus Toxoid and (CpG-ODN)

Author(s):

Mohsen Tafaghodi^{1, 2,*},

Seyed Abolghasem Sajadi Tabassi^{1, 3},

Mahmoud Reza Jaafari^{1, 4}

1School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

2Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

3Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran

4Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

IJ Pharmaceutical Research:Vol. 6, issue 3; 151-158

Published online:Nov 20, 2010

Article type:Research Article

Received:Sep 21, 2006

Accepted:Mar 21, 2007

How to Cite:Mohsen TafaghodiSeyed Abolghasem Sajadi TabassiMahmoud Reza JaafariNasal Immunization by (PLGA) Nanospheres Encapsulated with Tetanus Toxoid and (CpG-ODN).Iran J Pharm Res.6(3):e128326.https://doi.org/10.22037/ijpr.2010.715.

Abstract

In induction of systemic and mucosal immunity, particulate antigens are more effective than soluble antigens possibly because they are more efficiently endocytosed by mucosal-associated lymphoid tissue (MALT) M cells. In this study, we determined the systemic and mucosal immune responses in rabbits following intranasal immunization of tetanus toxoid TT and CpG-ODN encapsulated within PLGA nanospheres. The mean diameter of (TT) and TT+CpG nanospheres were 753±193 and 684±324 nm, respectively. Encapsulation efficiency of TT and CpG-ODN was determined as 52±7.8% and 30.2±7.4%, respectively. The highest nasal lavage (sIgA) titers were observed in groups immunized with nanosphere formulations, while the IgG and antitoxin titers were suppressed by these formulations. CpG-ODN as an adjuvant could increase the serum IgG and antitoxin titers when co-administered with TT solution or co-encapsulated with TT in PLGA nanospheres, but failed to potentiate the IgA titers in nasal lavages. No hemolysis was occurred on incubation of PLGA nanospheres and human (RBCs). Also after nasal administration of plain nanospheres to human volunteers, no local irritation was seen. Intranasal administration of nanospheres encapsulated with vaccines showed to be an effective way for inducing mucosal sIgA immune responses, and CpG-ODN could increase the systemic immune responses.

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Author(s):

Mohsen Tafaghodi:[PubMed][Scholar]
Seyed Abolghasem Sajadi Tabassi:[PubMed][Scholar]
Mahmoud Reza Jaafari:[PubMed][Scholar]