The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods

authors:

avatar Bardia Farzamfar 1 , avatar Saeed Bayanolhagh 2 , 3 , avatar Fereidoun Mahboudi 2 , avatar Mahin Zahrai 1 , *

Department of Medical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Biotechnology Process Development Center, Pasteur Institute of Iran, Tehran, Iran
Department of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran
Iranian Journal of Pharmaceutical Research: Vol.6, issue 3; 179-184
published online: November 20, 2010
article type: Research Article
received: May 21, 2006
accepted: November 21, 2006
DOI: https://doi.org/10.22037/ijpr.2010.719

how to cite: Farzamfar B, Bayanolhagh S, Mahboudi F, Zahrai M. The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods. Iran J Pharm Res. 2007;6(3):e128330. https://doi.org/10.22037/ijpr.2010.719.

Abstract

Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared conjugated molecule, Horseradish peroxidase-Bovine serum albumin- Aflatoxin B1 (HRP-BSA-AFB1). Stabilizing effects of six different stabilizers were compared during 10 months verification at room temperature and 6 weeks verification at 37°C. Based on the results, it was concluded that trehalose-containing stabilizers especially those contain casein in their compositions show the best stabilizing effects on HRP-BSA-AFB1 conjugated tracer.