The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods

Bardia Farzamfar; Saeed Bayanolhagh; Fereidoun Mahboudi; Mahin Zahrai

doi:10.22037/ijpr.2010.719

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.719

The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods

Author(s):

Bardia Farzamfar¹,

Saeed Bayanolhagh^{2, 3},

Fereidoun Mahboudi²,

Mahin Zahrai^1,*

1Department of Medical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2Biotechnology Process Development Center, Pasteur Institute of Iran, Tehran, Iran

3Department of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran

IJ Pharmaceutical Research:Vol. 6, issue 3; 179-184

Published online:Nov 20, 2010

Article type:Research Article

Received:May 21, 2006

Accepted:Nov 21, 2006

How to Cite:Bardia FarzamfarSaeed BayanolhaghFereidoun MahboudiMahin ZahraiThe Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods.Iran J Pharm Res.6(3):e128330.https://doi.org/10.22037/ijpr.2010.719.

Abstract

Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared conjugated molecule, Horseradish peroxidase-Bovine serum albumin- Aflatoxin B1 (HRP-BSA-AFB1). Stabilizing effects of six different stabilizers were compared during 10 months verification at room temperature and 6 weeks verification at 37°C. Based on the results, it was concluded that trehalose-containing stabilizers especially those contain casein in their compositions show the best stabilizing effects on HRP-BSA-AFB1 conjugated tracer.

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Author(s):

Bardia Farzamfar:[PubMed][Scholar]
Saeed Bayanolhagh:[PubMed][Scholar]
Fereidoun Mahboudi:[PubMed][Scholar]
Mahin Zahrai:[PubMed][Scholar]