Critical role of GSH in Sulfur Mustard-induced Oxidative Stress and Cytotoxicity in Human Skin Fibroblast Cell Line

Ali Beman Zaree Mahmoudabad; Mehdy Saberi; Jilla Pirzad

doi:10.22037/ijpr.2010.742

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.742

Critical role of GSH in Sulfur Mustard-induced Oxidative Stress and Cytotoxicity in Human Skin Fibroblast Cell Line

Author(s):

Ali Beman Zaree Mahmoudabad^1,*,

Mehdy Saberi²,

Jilla Pirzad²

1Department of Biochemistry and Molecular Biology Research Center and Chemical Injury Reasarch Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2Department of Pharmacology and Chemical Injury Reasarch Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

IJ Pharmaceutical Research:Vol. 7, issue 1; 35-41

Published online:Nov 20, 2010

Article type:Research Article

Received:Apr 01, 2007

Accepted:Sep 01, 2007

How to Cite:Ali Beman Zaree MahmoudabadMehdy SaberiJilla PirzadCritical role of GSH in Sulfur Mustard-induced Oxidative Stress and Cytotoxicity in Human Skin Fibroblast Cell Line.Iran J Pharm Res.7(1):e128568.https://doi.org/10.22037/ijpr.2010.742.

Abstract

In this study the role of glutathione (GSH) in sulfur mustard -induced oxidative stress and cytotoxicity, in human skin fibroblast cell line (HF2FF) was evaluated. Sulfur mustard-induced superoxide radical and hydrogen peroxide formation were evaluated by determination of superoxide dismutase and catalase activity in cell lysate. The cytotoxicity of sulfur mustard was estimated by lactate dehydrogenase leakage. The intracellular GSH content was modulated by N-acetylcysteine (NAC), a GSH precursor, and buthionine sulfoximine (BSO), a specific GSH synthesis inhibitor. It was found that sulfur mustard exposure led to a dose-and time-dependent decrease in GSH content in HF2FF cells. NAC increased intracellular GSH level and protected the cells against sulfur mustard-induced reactive oxygen species formation and lactate dehydrogenase leakage. In contrast, buthionine sulfoximine pretreatment depleted cellular GSH and enhanced the susceptibility of HF2FF to the cytotoxic effects of sulfur mustard. These results indicated that GSH plays a critical role in protecting HF2FF cell line against sulfur mustar-induced cell injury, most probably through its antioxidant activity.

Keywords

Buthionine sulfoximine

NAC

HF2FF cells

Sulfur mustard

Reactive oxygen species

Glutathione

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Author(s):

Ali Beman Zaree Mahmoudabad:[PubMed][Scholar]
Mehdy Saberi:[PubMed][Scholar]
Jilla Pirzad:[PubMed][Scholar]