Chiral Separation of Basic and Zwitterionic Drugs by Highly Sulfated Cyclodextrins Using Short-End Injection Capillary ElectrophoresisHighly Sulfated Cyclodextrins Using Short-End Injection Capillary Electrophoresis

authors:

avatar Majid Zandkarimi 1 , 2 , avatar Alireza Shafaati 1 , * , avatar Sayyed Mohsen Foroutan 1

School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
Iranian Journal of Pharmaceutical Research: Vol.7, issue 4; 275-281
published online: November 20, 2010
article type: Research Article
received: May 01, 2008
accepted: July 01, 2008
DOI: https://doi.org/10.22037/ijpr.2010.776

how to cite: Zandkarimi M, Shafaati A, Foroutan S M. Chiral Separation of Basic and Zwitterionic Drugs by Highly Sulfated Cyclodextrins Using Short-End Injection Capillary ElectrophoresisHighly Sulfated Cyclodextrins Using Short-End Injection Capillary Electrophoresis. Iran J Pharm Res. 2008;7(4):e128602. https://doi.org/10.22037/ijpr.2010.776.

Abstract

Due to high resolution power of sulfated cyclodextrins (HS-CDs), utilization of these selectors for chiral resolution of 7 basic and 2 zwitterionic drugs have been examined. Experiments were performed on a HP3DCE instrument equipped with an on-column diode array UV absorbance detector. Fused silica capillaries with an inner diameter of 50 µm, an outer diameter of 365 µm, and a total length of 45.5 cm (8.5 cm to the detector) were used. Capillaries were rinsed with PEO once daily. To reduce analysis time, short-end injection technique was applied. The electrophoretic conditions for the stereoselective analysis of drugs were in the carrier mode with 25 mM sodium phosphate buffer containing 1.25% w/v of each HS-CD at pH 2.5 with an applied voltage of +15 kV. At these conditions, rapid enantioresolution(?3.9 min) of all drugs were achieved.