Determination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran

Atefeh Bahmanzadegan; Fatemeh Sefidkon; Ali Sonboli

doi:10.22037/ijpr.2010.790

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.790

Determination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran

Author(s):

Atefeh Bahmanzadegan¹,

Fatemeh Sefidkon^1,*,

Ali Sonboli²

1Department of Phytochemistry, Research Institute of Forests and Rangelands, Tehran, Iran

2Department of Biology, Medicinal Plants and Drugs Research Institute, Shaheed Beheshti University, Tehran, Iran

IJ Pharmaceutical Research:Vol. 8, issue 1; 65-70

Published online:Nov 20, 2010

Article type:Research Article

Received:Feb 01, 2008

Accepted:Jun 01, 2008

How to Cite:Atefeh BahmanzadeganFatemeh SefidkonAli SonboliDetermination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran.Iran J Pharm Res.8(1):e128617.https://doi.org/10.22037/ijpr.2010.790.

Abstract

The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C₁₈ reversed phase column. An isocratic mixture of triethylammonium phosphate buffer (30 Mm, pH 6.2) and acetonitrile (75:25), was used as the eluent. Hyoscyamine and scopolamine were determined by the external standard method at 210 nm. All the four mentioned Hyoscyamus species contained hyoscyamine and scopolamine, but in different amounts. Scopolamine was the predominant tropane alkaloid in H. pusillus, H. niger and H. kurdicus, while, H. reticulatus contained a higher amount of hyoscyamine.

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Author(s):

Atefeh Bahmanzadegan:[PubMed][Scholar]
Fatemeh Sefidkon:[PubMed][Scholar]
Ali Sonboli:[PubMed][Scholar]