Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls

authors:

avatar Sadia Rehman 1 , * , avatar Nusrat Saba 1 , avatar Naveed Akhtar 2 , avatar Saeeda Munir 1 , avatar Waqar Ahmed 3 , avatar Azra Khanum 4

Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan
Shifa College of Medicine, Shifa Tameer-e-Millat University, Islamabad, Pakistan
Armed Forces Institute of Cardiology and National Institute of Heart diseases, Rawalpindi, Pakistan
Pir Mehr Ali Shah Arid Agriculture University, Rawlpindi, Pakistan
International Cardiovascular Research Journal: Vol.9, issue 3; e11238
published online: September 30, 2017
article type: Research Article
received: February 28, 2017
accepted: April 08, 2015

how to cite: Rehman S , Saba N , Akhtar N , Munir S , Ahmed W , et al. Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls. Int Cardiovasc Res J. 2015;9(3):e11238. 

Abstract

Background: Valve scarring and collagen deposition are crucial in pathogenesis of Rheumatic Heart Disease (RHD), an autoimmune disorder of the heart. Angiotensin I-Converting Enzyme (ACE) plays a major role in fibrous tissue formation.
Objectives: The present research work aimed to assess the role of ACE Insertion/Deletion (I/D) polymorphism in progress of RHD.
Patients and Methods: DNA was pre pared from blood samples from 156 RHD patients (156) and 204 healthy ethnically-matched controls. Then, it was screened using sequence-specific Primers. Polymerase chain reaction and Agarose gel electrophoresis. The data were analyzed using Vassar stats (http://faculty.vassar.edu/lowry/VassarStats.html).
Results: I allele (P = 0.024, OR = 1.42) and II genotype (P = 0.001, OR = 3.07) were significantly higher in Pakistani RHD patients compared to the healthy controls. Also, a significant difference was found between the female, but not male, patients and the controls regarding I allele and II genotype.
Conclusions: The study results provided information about involvement of ACE I/D polymorphism in molecular mechanism of RHD. Thus, it can become one of the useful tools in risk assessment and help with designing strategies to combat the disease.

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References

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