Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls

Sadia  Rehman; Nusrat  Saba; Naveed  Akhtar; Saeeda  Munir; Waqar  Ahmed; Azra Khanum

International Cardiovascular Research Journal

The Official Annually Journal of Cardiovascular Research Center, Shiraz University of Medical Sciences

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Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls

Authors:

Sadia Rehman^1,*,

Nusrat Saba¹,

Naveed Akhtar²,

Saeeda Munir¹,

Waqar Ahmed³,

Azra Khanum⁴

1Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan

2Shifa College of Medicine, Shifa Tameer-e-Millat University, Islamabad, Pakistan

3Armed Forces Institute of Cardiology and National Institute of Heart diseases, Rawalpindi, Pakistan

4Pir Mehr Ali Shah Arid Agriculture University, Rawlpindi, Pakistan

International Cardiovascular Research Journal:Vol. 9, issue 3; e11238

Published online:Sep 30, 2017

Article type:Research Article

Received:Feb 28, 2017

Accepted:Apr 08, 2015

How to Cite:Sadia RehmanNusrat SabaNaveed AkhtarSaeeda MunirWaqar AhmedAzra Khanumet al.Angiotensin Converting Enzyme Gene I/D Polymorphism in Pakistani Rheumatic Heart Disease Patients and Healthy Controls.9(3):e11238.

Abstract

Background: Valve scarring and collagen deposition are crucial in pathogenesis of Rheumatic Heart Disease (RHD), an autoimmune disorder of the heart. Angiotensin I-Converting Enzyme (ACE) plays a major role in fibrous tissue formation.

Objectives: The present research work aimed to assess the role of ACE Insertion/Deletion (I/D) polymorphism in progress of RHD.

Patients and Methods: DNA was pre pared from blood samples from 156 RHD patients (156) and 204 healthy ethnically-matched controls. Then, it was screened using sequence-specific Primers. Polymerase chain reaction and Agarose gel electrophoresis. The data were analyzed using Vassar stats (http://faculty.vassar.edu/lowry/VassarStats.html).

Results: I allele (P = 0.024, OR = 1.42) and II genotype (P = 0.001, OR = 3.07) were significantly higher in Pakistani RHD patients compared to the healthy controls. Also, a significant difference was found between the female, but not male, patients and the controls regarding I allele and II genotype.

Conclusions: The study results provided information about involvement of ACE I/D polymorphism in molecular mechanism of RHD. Thus, it can become one of the useful tools in risk assessment and help with designing strategies to combat the disease.

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