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The Effect of Human Cardiac Myocyte-Derived Conditioned Medium on Differentiation of Mesenchymal Stem Cells

AUTHORS

avatar Nadia Rousta ORCID 1 , avatar Sirous Naeimi 1 , avatar Shahrokh Zare 2 , avatar Mahintaj Dara ORCID 2 , avatar Iman Razeghian-Jahromi ORCID 3 , *

1 Department of Genetics, College of Science, Kazerun Branch, Islamic Azad University, Kazerun, IR Iran

2 Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran

3 Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran

How to Cite: Rousta N, Naeimi S, Zare S, Dara M, Razeghian-Jahromi I. The Effect of Human Cardiac Myocyte-Derived Conditioned Medium on Differentiation of Mesenchymal Stem Cells. Int Cardio Res J. 2022;16(3):e129983.

ARTICLE INFORMATION

International Cardiovascular Research Journal: 16 (3); e129983
Published Online: September 30, 2022
Article Type: Research Article
Received: July 16, 2022
Revised: August 17, 2022
Accepted: September 05, 2022
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Abstract

Background: Mesenchymal stem cells (MSC) possess specific properties that make
them good candidates for cell therapy, especially in organs like the heart with limited
regeneration capacity. Generating cardiac myocytes in vitro is an important step toward
restoring lost function in cardiovascular diseases.
Objective: In this study, we investigated the potential of a human cardiomyocytesconditioned
medium to induce cardiogenic differentiation (indicated by GATA4
expression) of rat bone marrow MSCs.
Methods: Rat bone marrow MSCs were extracted and cultured. They were characterized
based on morphology, adipogenic and cardiogenic differentiation potential, and
expression of mesenchymal markers. MSCs were exposed to the conditioned medium
(case group) or normal saline for 1, 3, and 7 days. The growth curve, population doubling
time, and GATA4 expression were compared between the groups.
Results: MSCs of the case group showed accelerated growth with a lower population
doubling time. GATA4 expression as a specific cardiac marker was significantly higher in
the case group compared with the controls on day 1. By days 3 and 7, GATA4 expression
remained higher, albeit the difference with the control group became less.
Conclusions: Our findings demonstrated that possible cardiac myocyte-derived factors
instigate the proliferation and cardiogenic differentiation of MSCs. This study provides a
novel bone marrow MSC model for investigating early cardiomyocyte generation, which
can open new avenues in the treatment of heart diseases.

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References

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© 2022, Shiraz University of Medical Sciences.
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